FIGURE SUMMARY
Title

Preparation of Paeonol Ethosomes by Microfluidic Technology Combined with Gaussians and Evaluation of Biological Activity by Zebrafish

Authors
Tian, M., Zhang, Z., Wang, L., Lei, F., Wang, Z., Ma, X., Gong, Z., Wang, J., He, J., Wang, D.
Source
Full text @ ACS Omega
Fig. 1

Effects of the single factor on the EE, particle size, and PDI of PAE-ethosomes. A and B: effects of total flow rate; C and D: effects of cholesterol concentration; E and F: effects of phospholipid concentration. Standard errors of the means (n = 3) are shown by the vertical bars. Duncan?s multiple range test indicates that values within each item that are indicated with distinct lower-case letters are substantially different (p < 0.05).

Fig. 2

Effects of various parameters on EE with 3-D response surface plots and 2-D contour plots.

Fig. 3

TEM results of PAE-ethosomes on particle size distributions and surface morphologies.

Fig. 4

FT-IR spectra of pyonosulfonyl alcohol, soybean-phospholipid (PC), cholesterol (CHO), mannitol, and PAE-ethosome.

Fig. 5

Effect on EE, ZP, VS, and PDI stored at different temperatures: 4 and 25 ± 1 °C for 30 days (n = 3).

Fig. 6

In vitro release curve of paeonol and paeonol ethosomes (n = 3).

Fig. 7

Soybean phospholipid interacted with paeonol via hydrogen bonds.

Fig. 8

In-vitro permeation profiles of paeonol formulations through the Bama minia ture pig skin (n = 3).

Fig. 9

Deposition of paeonol in the Bama minia ture pig skin after the in vitro skin permeation experiment (n = 3).

Fig. 10

In vivo anti-inflammatory activity in transgenic fluorescent zebrafish Tg (Lyz: EGFP JS7). Scale bar is 100 ?m. A: treated with lipid (40 ?g/mL), paeonol (5, 10, 20, 30, and 40 ?g/mL), and PAE-ethosomes (5, 10, 20, 30, and 40 ?g/mL). B: number of inflammatory cell migration in zebrafish after treatment with paeonol and PAE-ethosomes (5, 10, 20, 30, and 40 ?g/mL). One-way ANOVA and Dunnett?s test were used to evaluate the data using Graph Pad Prism 7.0 (GraphPad Software; CA, USA). The findings were presented as mean ± SD, ***p < 0.001 VS CuSO4, and ###P < 0.001 VS Ctrl.

Fig. 11

In vivo immunomodulatory effects in transgenic fluorescent zebrafish Tg (Lyz: EGFP). Scale bar is 100 ?m. A: treated with vinorelbine (150 ?g/mL), lipid (40 ?g/mL), paeonol (2.5, 5, 10, 20, 30, and 40 ?g/mL), and PAE-ethosomes (2.5, 5, 10, 20, 30, and 40 ?g/mL). B: number of macrophages in zebrafish after treatment with vinorelbine (150 ?g/mL), paeonol, and PAE-ethosomes (2.5, 5, 10, 20, 30, and 40 ?g/mL). The data were analyzed by one-way ANOVA followed by Dunnett?s test using Graph Pad Prism 7.0 (GraphPad Software; CA, USA). The results were expressed as mean ± SD, **P < 0.01, ***P < 0.001 VS Ctrl.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ ACS Omega
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