Effects of (A) homosalate (HS) and (B) octisalate (OS) on embryo coagulation, hatchability, and larval survival in wild-type (black circle), thr?a-/- (red circle), and miR-499-/- (green inverted triangle) zebrafish embryo/larvae. The results are shown as arithmetic mean ± standard deviation of a total of 72 embryo/larvae (24 larvae/replicate with 3 replicates). Asterisk (*) indicates significant differences between solvent control and exposure groups (p<0.05). Sharp (#) indicates significant differences between wild-type and knockout fish groups (p<0.05).

Effects on body length, body weight, triiodothyronine (T3), thyroxine (T4), thyroid-stimulating hormone (TSH) and transcriptional response of the genes involved in the hypothalamus-pituitary-thyroid (HPT) axis in zebrafish larvae exposed to (A) homosalate (HS) and (B) octisalate (OS) in wild-type zebrafish larvae. The results are shown as arithmetic mean ± standard deviation (n=4). Asterisk (*) indicates significant differences between control and exposure groups (p<0.05).

Effects on (A) growth hormone (GH), (B) insulin-like growth factor-1 (IGF-1), and (C) transcriptional response of the genes involved in the GH/IGF axis in zebrafish larvae exposed to homosalate (HS) and octisalate (OS) in wild-type zebrafish larvae. The results are shown as arithmetic mean ± standard deviation (n=4). Asterisk (*) indicates significant differences between control and exposure groups (p<0.05).