FIGURE SUMMARY
Title

Design and Application of Cu2+ Fluorescent Sensor Based on Carbazole Derivatives

Authors
Li, T., Xiao, X., Zhou, C., Luo, M.
Source
Full text @ J. Fluoresc.
Fig. 1

UV-vis absorption response of Sensor 1 in different concentrations of Cu2 + in HEPES − NaOH buffer (pH = 7.4) − DMF(1/1, v/v)

Fig. 2

Fluorescence spectrum of Sensor 1 in different metal ions in HEPES − NaOH buffer (pH = 7.4) − DMF(1/1, v/v) ( λex = 298 nm, slits: 2.5 nm/2.5 nm)

Fig. 3

Characterization of fluorescence quenching of Sensor 1 in different metal ions in HEPES − NaOH buffer (pH = 7.4) − DMF(1/1, v/v) at 365 nm in the fluorescence emission spectrum ( λex = 298 nm, λem = 365 nm, slits: 2.5 nm/2.5 nm)

Fig. 4

Histograms of fluorescence selective experiments (purple) and competitive experiments (green) of Sensor 1 in different metal ions in HEPES − NaOH buffer (pH = 7.4) − DMF(1/1, v/v) at 365 nm in the fluorescence emission spectrum ( λex = 298 nm, λem = 365 nm, slits: 2.5 nm/2.5 nm)

Fig. 5

Fluorescence response of Sensor 1 to Cu2+ in different concentrations in HEPES − NaOH buffer (pH = 7.4) − DMF(1/1, v/v) ( λex = 298 nm, λem = 365 nm, slits: 2.5 nm/2.5 nm)

Fig. 6

The linear fitting of the fluorescence titration curve of Sensor 1 in HEPES − NaOH buffer (pH = 7.4) − DMF(1/1, v/v) at 365 nm in the fluorescence emission spectrum ( λex = 298 nm, λem = 365 nm, slits: 2.5 nm/2.5 nm) and the combined model diagram between Sensor 1 and Cu2+

Fig. 7

Fluorescence spectra of 0.1 mM Sensor 1 in the presence of 0.1 mM Cu2+ and 0.3 mM EDTA in HEPES − NaOH buffer (pH = 7.4) − DMF(1/1, v/v) at 365 nm in the fluorescence emission spectrum ( λex = 298 nm, slits: 2.5 nm/2.5 nm)

Fig. 8

Fluorescence intensity of Sensor 1 ( 0.1 mM ) measured with and without Cu2+ ions ( 0.1 mM ) as a function of pH in HEPES − NaOH buffer (pH = 7.4) − DMF(1/1, v/v) at 365 nm in the fluorescence emission spectrum ( λex = 298 nm, λem = 365 nm, slits: 2.5 nm/2.5 nm)

Fig. 9

Concentration-dependent cell viability assay

Fig. 10

(a) Bright-field transmission image of HeLa cells treated with Sensor 1; (b) Fluorescence imaging of HeLa cells treated with Sensor 1;(c) Fluorescence imaging of HeLa cells treated with Sensor 1-Cu2+

Fig. 11

Images of zebrafish under UV light at 365 nm: (a) Zebra fish bred with only Sensor 1 for 1 h; (b) Fish bred with Sensor 1 and then bred with Cu2+ for 1 h

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Fluoresc.
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