FIGURE SUMMARY
Title

Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet

Authors
Liu, N., Zhang, P., Xue, M., Zhang, M., Huang, Z., Xu, C., Meng, Y., Fan, Y., Liu, W., Zhang, F., Chen, P., Zhou, Y.
Source
Full text @ Int. J. Mol. Sci.
Figure 1

Tocotrienol can reduce the triglyceride and cholesterol contents in zebrafish larvae. (A) Oil red O staining results. Scale: 1 mm. (B) Filipin staining results. Scale: 1 mm. (C) Quantitative results of the oil red O staining experiment. (D) Quantitative results of the Filipin staining experiment. ## represents p < 0.01 compared to the control group and * represents p < 0.05 compared to the HFD group.
Figure 2

Tocotrienol can reduce the contents of triglyceride and cholesterol in the liver of adult zebrafish. (A) The content of triglycerides in the liver of adult zebrafish. (B) Cholesterol content in the liver of adult zebrafish. Compared to the control group, ## means p < 0.01; compared to the HFD group, ** means p < 0.05.
Figure 3

Changes in intraocular blood vessels in the zebrafish lens. (A) Confocal scanning of isolated Tg (fli1: EGFP) zebrafish eye, showing intraocular blood vessels in the lens. Transparent vessels can be seen from the outside through the translucent lens (indicated by white arrows in A), and the diameter of the intraocular blood vessels to be measured is indicated by blue arrows. Scale bar: 10 μm. (B) Quantification of the diameter of intraocular blood vessels in confocal scanning images, n = 6. ## indicates comparison with the blank control group, with p < 0.01. * indicates comparison with the model group, with p < 0.05. ** indicates a significant difference compared to the model group, with p < 0.01.
Figure 4

The expression levels of the PPARγ and RXRα proteins in the adult zebrafish T2MD model. the expression levels of the PPARγ and RXRα proteins in the model group were significantly higher than those in the blank group, and the expression levels of the PPARγ and RXRα proteins in the experimental group were significantly lower than those in the model group. ## indicates that, compared to the blank control group, p < 0.01; ** indicates that, compared to the model group, p < 0.01.
Figure 5

Liver lipid metabolism-related gene mRNA expression. # indicates that, compared to the blank control group, p < 0.05; ## indicates that, compared to the blank control group, p < 0.01; * indicates that, compared to the model group, p < 0.05; ** indicates that, compared to the model group, p < 0.01.
Figure 6

Results of the lipid analysis in the adult zebrafish liver. (A) Correlation analysis among the control group, HFD group, and tocotrienol group. (B) PCA analysis results, where the x-axis represents the first principal component and the y-axis represents the second principal component. (C) The TOP20 detected lipid classification results.
Figure 6

Results of the lipid analysis in the adult zebrafish liver. (A) Correlation analysis among the control group, HFD group, and tocotrienol group. (B) PCA analysis results, where the x-axis represents the first principal component and the y-axis represents the second principal component. (C) The TOP20 detected lipid classification results.
Figure 7

Differential lipid analysis between the HFD group and tocotrienol group. (A) OPLS-DA score plot. (B) TOP20 differential lipid KEGG annotation results. (C) Volcano plot results. (D) Differential lipid clustering heat map.
Figure 7

Differential lipid analysis between the HFD group and tocotrienol group. (A) OPLS-DA score plot. (B) TOP20 differential lipid KEGG annotation results. (C) Volcano plot results. (D) Differential lipid clustering heat map.
Figure 8

(A) Differential lipid KEGG enrichment factor bubble diagram. (B) Difference multiple histograms.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.
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