C. procera leaf extract inhibited the growth of Glioblastoma cell lines Three Glioblastoma cell lines (U87 GM, U251 GM and A1235) were treated with serial dilutions (0.5.1.0,2.5,5.0,7.0,10.0 μg/ml) of C. procera methanol extract for 48 h. The cell viability was evaluated by MTT cell viability assay. The bars represent the average value of three replicates and error bars are ± standard deviation between the replicates * denotes the degree of statistic difference between plant extract treated and mock control (methanol 0.5 % V/V) cells., **p value = 0.01, ***p value = 0.001

The antiproliferation potential of Trichostatin A (TSA) in GBM cell lines TSA was used as positive control to compare the effect of C. procera, and TSA on cell viability of Glioblastoma cell lines. Three Glioblastoma cell lines (U87 GM, U251 GM and A1235) were treated with serial dilution (1.0, 1.5, 3.0 and 5 μg/ml) of TSA for 48 h X-values represents the concentration of extract and Y-values are percent cell mortality compared with control (mock treated cells). The bars represent the average value of three replicates and error bars are ± standard deviation between the replicates * denotes the degree of statistic difference between plant extract treated and mock treated cell lines. **p value = 0.01, ***p value = 0.001.

C. procera methanol extract of leaves induced cell cycle arrest in GBM Cells.

In vivo developmental toxicity screening of C. procera methanol extract of leaves in zebrafish embryos.

In vivo anti-angiogenesis activity of C. procera leaf extract in Tg (fli1:EGFP) embryos at 72hpf.

The semaxanib and Trichostatin A exert an anti-angiogenic effect and also induce several embryonic abnormities in Tg (fli1:EGF) embryos

Antiangiogenic protein profiling related to C. procera in GBM cells.

Differential expression of HDAC isoforms regulation by C. procera in U251 cell line.