The expression, potential functions, diagnostic value and prognostic value of ARGs in OC patients. A The mRNA expression of ARGs in OC samples compared to normal ovary samples based on the TCGA database. B The diagnostic value of ARGs for OC patients. C The PPI network for ARGs. D The correlation among these ARGs in OC patients. E GO and F KEGG enrichment analysis for these ARGs. G ANGPTL4 protein expression in OC and normal ovaries based on the HPA database. H The prognostic value of ANGPTL4 for OC patients based on the TCGA database. I The expression of ANGPTL4 in normal ovary samples and benign and malignant ovarian disease samples. J GSEA of ANGPTL4 based on the TCGA database. K Immune infiltration analysis for OC patients according to ANGPTL4 expression. L The expression of ANGPTL4 in multiple OC cell lines. M The expression of ESM1 and ANGPTL4 in multiple OC cell lines by Western blot. *P < 0.05, **P < 0.01, ***P < 0.001

The effects of the ANGPTL4/JAK2/STAT3 pathway on OC proliferation, migration and invasion. A The protein levels of ANGPTL4, p-JAK2, JAK2, p-STAT3, STAT3, Vimentin, PCNA and hypoxia-inducible factor alpha (HIF-α) were detected in the NC-SKOV3, Vector-SKOV3, ANGPTL4 KD-SKOV3, ANGPTL4 KD-SKOV3 with Colivelin, NC-HeyA8, Vector-HeyA8, ANGPTL4 OE-HeyA8, and ANGPTL4 OE-HeyA8 with AG490 groups by Western blotting. B A workflow for transfecting cells, collecting conditioned medium, and conducting analyses on OC cells and HUVECs. The effect of ANGPTL4, ANGPTL4 + AG490, ANGPTL4 KD, and ANGPTL4 KD + Colivelin on Hey-A8 and SKOV3 cell proliferation ability via MTT (C) and EdU (D) analysis. The migration and invasion abilities of ANGPTL4, ANGPTL4 + AG490, ANGPTL4 KD, and ANGPTL4 KD + Colivelin were measured by wound healing (E) and Transwell invasion assays (F) in Hey-A8 and SKOV3 cells, respectively. *P < 0.05, **P < 0.01, ***P < 0.001

ANGPTL4 promotes OC growth and angiogenesis by activating the JAK2/STAT3 pathway in vivo. A Morphological observation and HE and IHC staining of xenografts in the NC-SKOV3, Vector-SKOV3, ANGPTL4 KD-SKOV3, ANGPTL4 KD-SKOV3 with Colivelin, NC-HeyA8, Vector-HeyA8, ANGPTL4 OE-HeyA8, and ANGPTL4 OE-HeyA8 with AG490 groups. B The tumor weight and volume and the IHC staining score of xenografts in the NC-SKOV3, Vector-SKOV3, ANGPTL4 KD-SKOV3, ANGPTL4 KD-SKOV3 with Colivelin, NC-HeyA8, Vector-HeyA8, ANGPTL4 OE-HeyA8, and ANGPTL4 OE-HeyA8 with AG490 groups. C The proliferation and tube formation of HUVECs cultured with the CM of HeyA8 cells were tested by EdU staining and tube formation assays. The chick chorioallantoic membrane assay measured the angiogenesis ability of HeyA8 CM in vivo. Angiogenesis ability of HeyA8 cells in a zebrafish model. The experimental groups were NC, Vector, ANGPTL4 OE and ANGPTL4 + AG490. D The proliferation and tube formation of HUVECs cultured with the CM of SKOV3 cells were tested by EdU staining and tube formation assays. The chick chorioallantoic membrane assay measured the angiogenesis ability of SKOV3 CM in vivo. Angiogenesis ability of SKOV3 cells in the zebrafish model. The experimental groups were NC, Vector, ANGPTL4 KD and ANGPTL4 KD + Colivelin. *P < 0.05, **P < 0.01, ***P < 0.001

ANGPTL4 interacted with ESM1. A The correlation expression of ESM1 and ANGPTL4 in OC samples based on TCGA database. B IF staining for ANGPTL4 and ESM1 in OC tissue samples, SKOV3 cell lines, HeyA8 cell lines and HUVECs. C The interaction of ANGPTL4 and ESM1 in HeyA8 cells by Co-IP analysis. D GST pull-down analysis for GST-ANGPTL4 and His-ESM1. E Molecular docking for ANGPTL4 and ESM1 by HADDOCK.

ESM1 promotes ANGPTL4 to combine with LPL by accelerating proliferation, invasion and lipid accumulation in OC cells. A Molecular docking for ANGPTL4, LPL and ESM1 by HADDOCK. B Co-IP showed the effects of ESM1 on the interaction between ANGPTL4 and LPL in HeyA8 and SKOV3 cells. The effect of vector, ANGPTL4, ANGPTL4 + ESM1, ANGPTL4 KD, and ANGPTL4 KD + ESM1 on Hey-A8 and SKOV3 cell lipid levels and proliferation ability via oil red O staining (C), MTT (D) and EdU (E) analysis. The migration and invasion abilities were measured by wound healing (F) and Transwell assays (G) in Hey-A8 and SKOV3 cells transfected with vector, ANGPTL4, ANGPTL4 + ESM1, ANGPTL4 KD, and ANGPTL4 KD + ESM1. *P < 0.05, **P < 0.01, ***P < 0.001

ESM1 inhibits ANGPTL4 binding to integrin α5β1 and VE-cadherin, which represses HUVEC proliferation and migration to induce vascular permeability in the tumor microenvironment. A Co-IP showed that ESM1 inhibited ANGPTL4 binding to α5β1 and VE-cadherin in HUVECs cultured with HeyA8 and SKOV3 cells, respectively. The effect of vector, ANGPTL4, ANGPTL4 + ESM1, ANGPTL4 KD, and ANGPTL4 KD + ESM1 on HUVECs cultured with Hey-A8 and SKOV3 cell lipid levels and proliferation ability via oil red O staining (B), MTT (C) and EdU (D) analysis. The migration ability was measured by wound healing (F) and Transwell assays (G) in HUVECs cultured with Hey-A8 and SKOV3 cells transfected with vector, ANGPTL4, ANGPTL4 + ESM1, ANGPTL4 KD, and ANGPTL4 KD + ESM1. *P < 0.05, **P < 0.01, ***P < 0.001

A model of the molecular mechanism by which ANGPTL4 promotes OC development and progression. A OC is a type of tumor with a rich blood supply. B There are many angiogenic factors in the tumor microenvironment of OC. C A variety of cells in the tumor microenvironment can take up these angiogenesis factors in a variety of ways to produce different pathophysiological effects. D ANGPTL4 can promote the proliferation and migration of OC cells by activating the JAK-STAT3 pathway, and ESM1 can bind ANGPTL4 and stabilize the binding of ANGPTL4 to LPL, thus promoting lipid accumulation and accelerating the proliferation and migration of OC cells. ANGPTL4 can also promote endothelial cell proliferation and migration through the JAK-STAT3 signaling pathway. ESM1 can inhibit the binding of ANGPTL4 to integrin α5β1 to increase vascular stability. Moreover, ESM1 promotes angiogenesis in the ovarian cancer microenvironment by binding to ANGPTL4 to inhibit its destruction of VE-cadherin-mediated tight junctions. E The molecular mechanism might be attributed to the interaction of ESM1 and ANGPTL4.