FIGURE SUMMARY
Title

Assessment of an Anticancer Effect of the Simultaneous Administration of MM-129 and Indoximod in the Colorectal Cancer Model

Authors
Kwiatkowska, I., Hermanowicz, J.M., Czarnomysy, R., Surażyński, A., Kowalczuk, K., Kałafut, J., Przybyszewska-Podstawka, A., Bielawski, K., Rivero-Müller, A., Mojzych, M., Pawlak, D.
Source
Full text @ Cancers
Figure 1

Impact of MM-129 (10 µM), IND (200 µM) or a combination of these agents (MM-129 + IND) on tumor development in DLD-1 (a), and HT-29 (b) zebrafish xenografts, and the expression of human GAPDH mRNA was determined at 120 hpf. Data were presented as mean ± standard deviation (SD) and analyzed using one-way analysis of variance (ANOVA). n = 20 each group; ** p < 0.01, *** p < 0.001 vs. CON; ^ p < 0.05, ^^^ p < 0.001 vs. MM-129; # p < 0.05 vs. IND.
Figure 2

Effect of MM-129 (10 µM), IND (200 µM), and compound combination (MM-129 + IND) on cell division in the zebrafish embryo. Zebrafish eggs after 0, 1, 1.25, and 2 h of exposure to tested compounds; n = 20, hpf: hours post-fertilization.
Figure 3

Viability of DLD-1 (a) and HT-29 (b) colon cancer cells treated for 24 h with MM-129 (10 µM), IND (200 µM), and compound combination (MM-129 + IND). Results are presented as mean values ± standard deviation (SD) from three independent experiments performed in duplicate. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CON; ### p < 0.001 vs. IND.
Figure 4

Representative dot-plots illustrating the loss of mitochondrial membrane potential, ΔΨm. (a,b) Flow cytometry dot-plots for the annexin V-FITC-propidium iodide assay (c,d) and quantitative chart illustrating the distribution of live, early, and late apoptotic and necrotic cells (e,f) of DLD-1 (a,c,e) and HT-29 (b,d,f) after 24 h exposition to MM-129 (10 µM), IND (200 µM), and MM-129 + IND. Values were obtained from three independent experiments.
Figure 5

Flow cytometric analysis of caspase-3/7, caspase-10, and caspase-8 activation in DLD-1 (a–c) and HT-29 (d–f) colon cancer cells exposed to MM-129 (10 µM), IND (200 µM), and MM-129 + IND for 24 h. Values from three independent experiments performed in duplicate were presented as mean ± standard deviation (SD) and analyzed using one-way analysis of variance (ANOVA). ** p < 0.01, *** p < 0.001 vs. CON; ^ p < 0.05, ^^ p < 0.01, ^^^ p < 0.001vs. MM-129; ### p < 0.001 vs. IND; && p < 0.01, &&& p < 0.001 IND vs. MM-129.
Figure 6

The downregulation of protein kinase B (AKT) (a,b) and indoleamine 2,3-dioxygenase-1 (IDO1) (c,d) exerted by MM-129 (10 µM), IND (200 µM), or MM-129 + IND toward DLD-1 and HT-29 colorectal cancer cells after 24 h exposition. The values were obtained from three independent experiments performed in duplicate. * p < 0.05, *** p < 0.001 vs. CON; ^^^ p < 0.01 vs. MM-129; ## p < 0.01, ### p < 0.001 vs. IND. Images and quantification were obtained by capillary protein separation and immunodetection. The uncropped blots are shown in the supplemental materials.
Acknowledgments
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