- Title
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Development of the integrated Fish Endocrine Disruptor Test (iFEDT) - Part A: Merging of existing fish test guidelines
- Authors
- Pannetier, P., Gölz, L., Pissarreira Mendes Fagundes, M.T., Knörr, S., Behnstedt, L., Coordes, S., Matthiessen, P., Morthorst, J.E., Vergauwen, L., Knapen, D., Holbech, H., Braunbeck, T., Baumann, L.
- Source
- Full text @ Integr. Environ. Assess. Manag.
The design of a new test protocol, the iFEDT. FSDT, fish sexual development test; FSTRA, fish short-term reproduction assay; iFEDT, integrated fish endocrine disruptor test; TG, test guideline; VTG, vitellogenin |
Cumulative number of eggs per female and day in adult zebrafish after 21-day exposure to (A) 6-propyl-2-thiouracil or (B) 17α-ethinylestradiol. Data are given as means ± SD (n = 19–24 per treatment, four replicates of 4–6 individuals each). Significant differences between treatment groups and controls after 21 days exposure: *p < 0.05, **p < 0.01, ***p < 0.001 |
Vitellogenin (VTG) levels in adult zebrafish after 21-day exposure to (A, B) PTU or (C, D) EE2. Data are given as means ± SD (n = 16–20 per treatment, four replicates of 3–7 individuals each). Significant differences between treatment groups and controls after 21-day exposure: *p < 0.05, **p < 0.01, ***p < 0.001. EE2, 17α-ethinylestradiol; PTU, 6-propyl-2-thiouracil |
Mean length and weight of juvenile F1 zebrafish exposed to different concentrations of (A, B) PTU or (C, D) EE2 for 60 days. Data are given as means ± SD (n = 78–100 per treatment, four replicates of 15–30 individuals each). Significant differences between treatment groups and controls after 21 days exposure: *p < 0.05, **p < 0.01, ***p < 0.001. EE2, 17α-ethinylestradiol; PTU, 6-propyl-2-thiouracil |
Vitellogenin (VTG) levels in juvenile F1 zebrafish exposed to different concentrations of (A, B) PTU or (C, D) EE2 for 60 days. Data are given as means ± SD (n = 26–72 per treatment, three replicates of 5–22 individuals each). Significant differences between treatment groups and controls after 21 days exposure: *p < 0.05, **p < 0.01, ***p < 0.001. EE2, 17α-ethinylestradiol; PTU, 6-propyl-2-thiouracil |
Sex ratio and gonad maturity index in juvenile F1 zebrafish exposed to different concentrations of (A, C) PTU or (B, D) EE2 for 60 days. Data are given as means ± SD (n = 18–92 per treatment). Significant differences between treatment groups and controls after 21 days exposure: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. EE2, 17α-ethinylestradiol; PTU, 6-propyl-2-thiouracil |