FIGURE SUMMARY
Title

Molecular characterization of adenosine monophosphate deaminase 1 and its regulatory mechanism for inosine monophosphate formation in triploid crucian carp

Authors
Zhou, Y., Zuo, A., Li, Y., Zhang, Y., Yi, Z., Zhao, D., Tang, J., Qu, F., Cao, S., Mao, Z., Jin, J., Liu, Z.
Source
Full text @ Front. Physiol.
FIGURE 1

The open reading frame (ORF) and amino acid sequences of AMPD1.
FIGURE 2

Adjacency phylogenetic tree based on AMPD1 amino acid sequences of different species. Node values represent the percent bootstrap confidence level derived from 1000 replicates. The bar (0.05) indicates the genetic distance.
FIGURE 3

The relative expression levels of AMPD1 gene in different tissues of triploid crucian carp were analyzed by real-time fluorescence quantitative PCR. Error bars represent the means ± SD; n = 3. p < 0.05.
FIGURE 4

Localization analysis of AMPD1 in cells. Scale bars represent 50 µm.
FIGURE 5

Effects of glutamate (Glu) on the relative mRNA expression of AMPD1 and the IMP content in vivo(A) and in vitro(B). Error bars represent the means ± SD; n = 3. p < 0.05.
FIGURE 6

Effects of AMPD1 inhibitor injection on IMP content and the relative mRNA expression of AMPK-mTOR signaling pathway related genes for 48 h and 72 h in vivo. (A) IMP content; (B) the relative mRNA expression of AMPK-mTOR signaling pathway related genes. Error bars represent the means ± SD; n = 3. p < 0.05.
FIGURE 7

Effects of 40 μM AMPD1 inhibitor on the IMP content and AMPK-mTOR signaling pathway related genes in vitro. (A) the sixth generation of muscle cells, (B) the sixth generation of muscle cells treated with 40 μM inhibitor for 16 h, bar = 100 μm, (C) the detection diagram of IMP content by HPLC; (D) the effects on the expression of AMPK-mTOR signaling pathway related genes. Error bars represent the means ± SD; n = 3. p < 0.05.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.
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