Fig. 1. Body length and weight of zebrafish (A and B) or marine medaka (C and D) at 6 mpf, after MG and PB MPs exposures. (Mean ± SD, Tukey HSD test, * indicates significant differences from respective control of same sex with * p < 0.05; **p < 0.001; ***p < 0.0001; n = 11 to 53 per sex and per treatment).

Fig. 2. Relative proportions of successful (white) and failed (black) attempts to obtain eggs from zebrafish pairs exposed to MG or PB MPs. (Fisher's exact test; ***: p < 0.0001. Attempts n = 56–66 from three replicates).

Fig. 3. Reproductive output of marine medaka exposed to MG or BP MPs. The number of eggs per day normalised according to the number of females present in the tank. R square of linear regressions are all above 0.9 (***: p < 0.0001; Attempts n = 18–38 from three replicates).

Fig. 4. Individual standard length of F1 larvae of adults zebrafish (left) and marine medaka (right) exposed to MG or PB MPs relative to control. (Mean ± SD; ANOVA; *: p < 0.05; **p < 0.01; zebrafish n = 9–15; marine medaka n = 44–60).

Fig. 5. Larval photomotor response in F1 generation, (A) 5 dpf zebrafish and (B) 14 dpf marine medaka after parental exposure to MG and PB MPs. Distance travelled over 5 min periods including two light-on periods (LON1, LON2) and one light off period (LOFF). (Mean ± SD repeated–measure ANOVA; differences between treatments within the same observation period as *p < 0.05; ***p < 0.001; n = 50 to 60 larvae per treatment).