FIGURE SUMMARY
Title

The Annotation of Zebrafish Enhancer Trap Lines Generated with PB Transposon

Authors
Jia, W., Guan, Z., Shi, S., Xiang, K., Chen, P., Tan, F., Ullah, N., Diaby, M., Guo, M., Song, C., Gao, B.
Source
Full text @ Curr. Iss. Mol. Biol.
Fig. 2

GFP expression patterns in the EYE line generated by the PB-mediated enhancer trap. (A–I) nine developmental stages. The arrows indicate GFP+ tissues.
Fig. 3

Electrophoresis image of PCR products in agarose gel. M: DL2000 bp marker; Line 1: the PCR product amplified from wild-type zebrafish genome by using GFP-F/R primers; Line 2: the PCR product amplified from EYE lines genome by using GFP-F/R primers; Line 3: the PCR product amplified from wild-type zebrafish genome by using Krt4-F and GFP-R primers; 4: the PCR product amplified from EYE lines genome by using Krt4-F and GFP-R primers. White boxes indicate the target bands (720 bp in Line 2, 916 bp in Line 4).
Fig. 4

Enhancer distribution between 50 kb upstream and 50 kb downstream of the insertion site in the EYE lines genome. Genomic sequences from Danio rerio, Northern-pike, Nile-tilapia, Mummichog, Midas-cichlid, Mexican tetra, and Amazon-molly were analyzed in the VISTA browser. The red boxes represent three conserved non-coding sequences (CNS) that are predicted to be enhancers between itgav and zc3h15 genes. The red arrow indicates the insertion site of the ET vector. UTR: untranslated region.
Fig. 5

Itgav structural map. The red arrow indicates the insertion site of the ET vector. The black arrow indicates the orientation of the gene itgav. Numbers 1, 2, 3 indicate three conserved non-coding sequences (CNS) located upstream of itgav or downstream of zc3h15. UTR: untranslated region; CNS: conserved non-coding sequence.
Fig. 6

In situ hybridization for itgav transcripts at different stages of wide type zebrafish embryos. Red arrows indicate similar expression patterns to GFP in the EYE line. Experimental group: zebrafish embryos treated with itgav antisense RNA probes. Control group: zebrafish embryos without probe treatment.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Iss. Mol. Biol.
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