Figure 1. Effect of exposure to [C8mim]Cl (5.08, 10.16, and 20.32 mg L⁻¹) for 96 h on the embryonic development of zebrafish. (A) The survival rate of zebrafish (96 h); (B) hatching rate of zebrafish after exposure for 48 h; (C) heart rate of zebrafish after exposure for 96 h; and (D) alteration of zebrafish body length after exposure (96 h). The results are represented as the means ± SEMs of three replicates. * p < 0.05, ** p < 0.01 expressed significant differences between the treatment group and the control group.

Figure 2. The mRNA expression levels of the hypothalamus–pituitary–thyroid axis genes in zebrafish embryos and larvae after exposure to 5.08, 10.16, and 20.32 mg L⁻¹ of [C8mim]Cl for 96 h. The results are represented as the means ± SEMs of three replicates. * p < 0.05, ** p < 0.01 express significant differences between the treatment group and control group.

Figure 3. The mRNA expression levels of the thyrotrophin-releasing hormone receptor family genes in zebrafish embryos and larvae exposed to 5.08, 10.16, and 20.32 mg L⁻¹ of [C8mim]Cl for 96 h. The results represented the means ± SEMs of three replicates. * p < 0.05, ** p < 0.01 express significant differences between the treatment group and control group.

Figure 4. Effect of [C8mim]Cl exposure on the thyroid hormone levels of zebrafish larvae after 96 h. (A) T3 hormone, (B) T4 hormone, and (C) the ratio of T3 to T4. * p < 0.05, ** p < 0.01 express significant differences between the treatment group and control group.