FIGURE SUMMARY
Title

Effects of Abelmoschus manihot Flower Extract on Enhancing Sexual Arousal and Reproductive Performance in Zebrafish

Authors
Chang, C.C., Houng, J.Y., Peng, W.H., Yeh, T.W., Wang, Y.Y., Chen, Y.L., Chang, T.H., Hung, W.C., Yu, T.H.
Source
Full text @ Molecules
Figure 1

Figure 1. Effects of the supplementation with AME on the chasing number between the male and female zebrafish: Each dataset is represented by five independent repeated experiments. Statistical differences compared to the vehicle group were tested using one-way ANOVA. Levels of significance are expressed as ** p < 0.01 and *** p < 0.001.

Figure 2

Figure 2. Effects of the supplementation of AME on the fertilized egg production. Each dataset is represented by five independent repeated experiments. Statistical differences compared to the vehicle group were tested using one-way ANOVA. Levels of significance are expressed as * p < 0.05, ** p < 0.01, and *** p < 0.001.

Figure 3

Figure 3. Effects of the supplementation with AME on the hatching rate of the fertilized eggs. Each dataset is represented by five independent repeated experiments. Statistical differences compared to the vehicle group were tested using one-way ANOVA. Levels of significance are expressed as ** p < 0.01 and *** p < 0.001.

Figure 4

Figure 4. Effects of the supplementation with AME (A: Female, B: Male) on the body weight of zebrafish during 42-day cultivation. At each sampling time, five male or female adult fish were weighed.

Figure 5

Figure 5. Effects of AME stimulation on the expression of sex-related genes in zebrafish: (A) female, lhcgr in ovaries; (B) female, cyp19a1a in the brain; (C) male, ar in the testes; (D) male, cyp19a1b in the brain. Five adult zebrafish were used for each experimental group at each sampling time. Statistical differences in gene expression between the AME-supplemented-diet group and the vehicle group were tested using one-way ANOVA, and levels of significance are denoted as * p < 0.05, ** p < 0.01, and *** p < 0.001.
Figure 6

Figure 6. HPLC chromatograms of AME: Identified components—rutin, hyperoside, isoquercitrin, myricetin, and quercetin. The peak of the internal standard (naringin) is denoted as “IS”.

Acknowledgments
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