FIGURE SUMMARY
Title

Protocol for inducing inflammation and acute myelin degeneration in larval zebrafish

Authors
Jaronen, M., Wheeler, M.A., Quintana, F.J.
Source
Full text @ STAR Protoc
Fig. 1

Expected outcomes of screening environmental factors in zebrafish

(A) Expression of myelin protein zero (mpz) in different exposure time points. LPS + cuprizone exposure results in significant demyelination in all measured time points.

(B) LPS + cuprizone induces expression of inflammatory markers. Expression of il1b, il10, il17a/f1, and ccl20 is significantly increased after 48 h cuprizone exposure.

(C) Expression of inducible nitric oxide synthase (nos2a) in different exposure time points.

(D) LPS + cuprizone causes astrocyte activation. Dot plot analysis of gfap::EGFP expression after 48 h LPS + cuprizone exposure demonstrates increased gfap::EGFP expression. Expression of nos2a in gfap::EGFP+ cells is increased after 48 h LPS + cuprizone exposure.

(E and F) Example upload and (F) visualization in NetworkAnalyst of geneset identified by compound screening. ∗∗p<0.001; ∗∗∗ p<0.0001. Data shown as mean±SEM.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ STAR Protoc
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