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Fig. 1 Expected outcomes of screening environmental factors in zebrafish
(A) Expression of myelin protein zero (mpz) in different exposure time points. LPS + cuprizone exposure results in significant demyelination in all measured time points.
(B) LPS + cuprizone induces expression of inflammatory markers. Expression of il1b, il10, il17a/f1, and ccl20 is significantly increased after 48 h cuprizone exposure.
(C) Expression of inducible nitric oxide synthase (nos2a) in different exposure time points.
(D) LPS + cuprizone causes astrocyte activation. Dot plot analysis of gfap::EGFP expression after 48 h LPS + cuprizone exposure demonstrates increased gfap::EGFP expression. Expression of nos2a in gfap::EGFP+ cells is increased after 48 h LPS + cuprizone exposure.
(E and F) Example upload and (F) visualization in NetworkAnalyst of geneset identified by compound screening. ∗∗p<0.001; ∗∗∗ p<0.0001. Data shown as mean±SEM.