FIGURE SUMMARY
Title

Vitamin D deficiency promotes accumulation of bioactive lipids and increased endocannabinoid tone in zebrafish

Authors
Knuth, M.M., Stutts, W.L., Ritter, M.M., Garrard, K.P., Kullman, S.W.
Source
Full text @ J. Lipid Res.
Fig. 1

Comparison of ROI lipid profiles from a VDD and VDS zebrafish. Mirror plots from full-scan MS1 spectra illustrate the chemical diversity and changes in lipid composition observed in VDD fish.
Fig. 2

Zebrafish orientation. A: All fish shown in IR-MALDESI-MSI images are presented in anterior to posterior and dorsal to ventral orientation. B: Representative histology image of a VDD fish with the visceral cavity (liver, intestines, spleen, and kidney) labeled.
Fig. 3

MSiReader images representing a select group of EC precursors detected in negative ionization mode. Fish are in anterior to posterior, dorsal to ventral orientation. Each image contains two male zebrafish at 6 mpf: a VDD fish (left) followed by a VDS fish (right). All ions were significantly (∗P ≤ 0.1; ∗∗P ≤ 0.05) more abundant (2-fold or greater) in VDD fish than VDS fish. ∗See also Table 1.
Fig. 4

MSiReader images representing a select group of EC ions detected in positive ionization mode. Fish are in anterior to posterior and dorsal to ventral orientation. Each image contains two male zebrafish at 6 mpf: a VDD fish (left) followed by a VDS fish (right). All ions were significantly (∗P ≤ 0.1; ∗∗P ≤ 0.05) more abundant (2-fold or greater) in VDD fish than VDS fish. ∗See also Table 2.
Fig. 5

MSiReader images representing a select group of sterols in positive ionization mode. Fish are in anterior to posterior and dorsal to ventral orientation. Each image contains two male zebrafish at 6 mpf: a VDD fish (left) followed by a VDS fish (right). All ions were significantly (∗P ≤ 0.1; ∗∗P ≤ 0.05) more abundant (2-fold or greater) in VDD fish than VDS fish. ∗See also Table 2.
Fig. 6

Evaluating AEA signaling, synthesis, and metabolism in VDD AT and Liver. A: VDD fish had significantly attenuated trpv1 and cnr2 expression and attenuated expression of cnr1 in AT compared with VDS fish. B: VDD fish had significantly increased napepld, abh4, and mgll expression in AT compared with VDS fish but significantly decreased daglb expression in liver. C: VDD fish had significantly increased faah1 and faah2a expression in AT compared with VDS fish.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Lipid Res.
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