FIGURE SUMMARY
Title

Apatinib inhibits the proliferation of gastric cancer cells via the AKT/GSK signaling pathway in vivo

Authors
Chen, Y., Chen, N., Xu, J., Wang, X., Wei, X., Tang, C., Duanmu, Z., Shi, J.
Source
Full text @ Aging (Albany NY)
Figure 1

The inhibition effect of apatinib on the cell viability in different GC cell lines. (A) Cell viability of different human gastric cancer cell lines treated with different concentrations of apatinib for 72 h. (B) After treatment with apatinib 2 μM concentration for 72 h, western blot analysis of AKT, phosphorylated AKT (p-AKT), GSK3α, phosphorylated GSK3α (p-GSK3α), GSK3β and phosphorylated GSK3β (p-GSK3β) in GC cell lines. (C) Cell viability of different human gastric cancer cell lines treated with different concentrations of apatinib for 8 h. (D) After treatment with apatinib 2 μM concentration for 8 h, western blot analysis of AKT, phosphorylated AKT (p-AKT), GSK3α, phosphorylated GSK3α (p-GSK3α), GSK3β and phosphorylated GSK3β (p-GSK3β) in GC cell lines.
Figure 2

Gastric cancer cell MGC-803 promoted angiogenesis in transgenic zebrafish tg (fli1a-EGFP). (A) Biologic morphology of zebrafish. Scale bar: 200 μm. (B) Fluorescence image of subintestinal vessels of the uninjected embryo. Scale bar: 50 μm. (C) Gastric cancer cell lines were injected to the zebrafish embryo, MGC-803 was taken as an example in the figure. Scale bar: 200 μm. (D) MGC-803 cells promoted angiogenesis at 1 dpi compared with control group. The white arrow indicated the tumor cell induced angiogenesis, dpi: days post injection. Scale bar: 50 μm. (E) Quantitative analysis of the length of newly formed vessels in zebrafish with/without inducing by MGC-803.
Figure 3

Inhibitory effect of gastric cancer cell line in the zebrafish xenograft model under apatinib treatment. (A) The survival rates and teratogenic rates of zebrafish were detected under different concentrations of apatinib. (B) Fluorescence image of subintestinal vessels in zebrafish induced by MGC-803 with/without apatinib treatment at 1 dpt. (C) Quantitative analysis of the length of newly formed vessels in zebrafish induced by MGC-803 with/without apatinib treatment. Scale bar: 50 μm. (D) Quantitative analysis of the MGC-803 cells proliferation with/without apatinib treatment at 2 dpt. Dpt: days post treatment. (E) Western blot analysis of AKT, phosphorylated AKT (p-AKT), GSK3α, phosphorylated GSK3α (p-GSK3α), GSK3β and phosphorylated GSK3β (p-GSK3β) in injected cells.
Figure 4

Proliferation of zebrafish xenograft model inhibited by apatinib treatment. (A) Fluorescence image of tumor size in zebrafish induced by MGC-803 with/without apatinib treatment at 1, 2 and 3 dpt. Scale bar: 200 μm. (B) Quantitative analysis of the length of MGC-803 cells proliferation in zebrafish with/without apatinib treatment. Dpt: days post treatment.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Aging (Albany NY)
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