FIGURE SUMMARY
Title

Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

Authors
Demin, K.A., Kolesnikova, T.O., Galstyan, D.S., Krotova, N.A., Ilyin, N.P., Derzhavina, K.A., Levchenko, N.A., Strekalova, T., de Abreu, M.S., Petersen, E.V., Seredinskaya, M., Cherneyko, Y.V., Kositsyn, Y.M., Sorokin, D.V., Zabegalov, K.N., Mor, M.S., Efimova, E.V., Kalueff, A.V.
Source
Full text @ Sci. Rep.
Figure 1

A brief diagram outlining the study experimental design, including the prolonged 11-week chronic unpredictable stress (PCUS) protocol and behavioral testing battery (see Table 1 for details of the PCUS stressors applied in the present study). Abbreviations: NTT the novel tank test, CPA the conditioned place aversion test, SH shoaling test, EPA eicosapentaenoic acid, LPS lipopolysaccharide, HPLC high-performance liquid chromatography.
Figure 2

Behavioral alterations induced by prolonged chronic unpredictable stress (PCUS) exposure and fluoxetine, EPA, or LPS treatment in adult zebrafish tested in the novel tank test. Data are presented as median and Q1, Q3 (n = 12–22 per group). *p < 0.05, **p < 0.01, ***p < 0.001 vs. control, post-hoc Dunn’s test for significant Kruskal–Wallis data. Graphs were constructed using the ggplot2 R package30 (also see Table 2 and Supplementary Table S1 for statistical details). Groups: C control, S PCUS, F fluoxetine, E eicosapentaenoic acid (EPA), L lipopolysaccharide (LPS).
Figure 3

Behavioral alterations induced by prolonged chronic unpredictable stress (PCUS) exposure and fluoxetine, EPA, or LPS treatment in adult zebrafish tested in the shoaling test (ST; inter-fish distance) and conditioned place avoidance (CPA, time spent in the light compartment). Data are presented as median and Q1, Q3 (total number of still images: 198–541 per group for ST (calculated as distances between each zebrafish in each captured still image, thus representing the number of images taken), n = 12–22 per group for CPA). *p < 0.05, **p < 0.01, ***p < 0.001 vs. control, post-hoc Dunn’s test for significant Kruskal–Wallis data. Graphs were constructed using the ggplot2 R package30, also see Table 2 and Supplementary Table S1 for statistical details). Groups: C control, S PCUS, I intact (no learning), F fluoxetine, E eicosapentaenoic acid (EPA), L lipopolysaccharide (LPS).
Figure 4

Neurochemical alterations induced by the prolonged chronic unpredictable stress (PCUS) exposure and fluoxetine, EPA, or LPS treatment in adult zebrafish brain assessed using HPLC. Data are presented as median and Q1, Q3 (n = 12 per group). *p < 0.05, **p < 0.01 vs. control, post-hoc Dunn’s test for significant Kruskal–Wallis data. Graphs were constructed using the ggplot2 R package30, also see Table 2 and Supplementary Table S2 for statistical details. Groups: C control, S PCUS, F fluoxetine, E eicosapentaenoic acid (EPA), L lipopolysaccharide (LPS).

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.
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