FIGURE SUMMARY
Title

Hybrid between Danio rerio female and Danio nigrofasciatus male produces aneuploid sperm with limited fertilization capacity

Authors
Endoh, M., Shima, F., Havelka, M., Asanuma, R., Yamaha, E., Fujimoto, T., Arai, K.
Source
Full text @ PLoS One
Fig 1

PCR-RFLP analysis of <italic>rhodopsin</italic> gene.

(Lanes 1 and 12) Molecular size marker. (Lanes 2–4) D. rerio gave a single fragment of 617 bp. (Lanes 5–7) The hybrids between D. rerio female and D. nigrofasciatus male possessed three fragments of 617, 365 and 252 bp. (Lanes 8–10) D. nigrofasciatus gave two fragments of 365 and 252 bp. (Lane 11) Negative control.
Fig 2

External appearance and histological images of ovaries.

(A, C) D. rerio, (B, D) the hybrid between a D. rerio female and a D. nigrofasciatus male. Scale bars indicate 1 mm (A and B) and 100 μm (C and D).
Fig 3

External appearance and histological images of testis.

(A, C) D. rerio, (B, D and E) the hybrid between D. rerio female and D. nigrofasciatus male. (C) Lumen in the control D. rerio testis was fully filled with mature spermatozoa. (D) A small number of mature spermatozoa was observed in the lumen of the hybrid male that generated aneuploid sperm. (E) There were no mature spermatozoa in the lumen of hybrid males that did not produce sperm. Scale bars indicate 1 mm (A and B) and 50 μm (C–E).
Fig 4

Morphology of spermatozoa.

(A) D. rerio, (B) the hybrid between D. rerio female and D. nigrofasciatus male. Spermatozoa morphology of D. rerio and the hybrid between D. rerio female and D. nigrofasciatus male. Head diameter of hybrid spermatozoa were 2.12 μm (solid arrow), 3.23 μm (solid arrowhead) and 4.41 μm (open arrowhead). Scale bars indicate 50 μm.
Fig 5

Box plot showing spermatozoa head diameter (μm).

(C1–C3) D. rerio males, (H1–H10) hybrids between D. rerio female and D. nigrofasciatus male. Red dashed lines delimit the smallest and the largest spermatozoa head diameter in D. rerio males. Each dot represents one measured spermatozoon. Number of analyzed spermatozoa in brackets.
Fig 6

Relative DNA contents measured by flow cytometry of fin-clips (A, B) and sperm (C, D). (A, C) D. rerio diploid control, (B, D) the hybrid between D. rerio female and D. nigrofasciatus male. Square bracket indicates range of aneuploid spermatozoa up to about diploidy.
Fig 7

External appearance of backcross progeny with various ploidy or aneuploidy levels.

(A) 1n larva, (B) 1.3n larva, (C) 1.5n larva, (D) 1.8n larva, (E) 2n larva, (F) 2.3n larva, (G) 2.5n larva, (H) 3n larva without blood circulation, and (I) 2.5n larva with normal appearance. All larvae were progeny from fertilization between D. rerio female and the interspecific hybrid male at 48 hours post fertilization. Scale bar indicates 1 mm.
Fig 8

Two-month-old backcross progeny from fertilization between <italic>D</italic>. <italic>rerio</italic> female and the hybrid male.

(A) D. rerio diploid control, (B) surviving triploid backcross progeny with normal appearance. Scale bars indicate 1 mm.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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