Effect of mir-20b on zebrafish embryos. (A) Injection of 4uM dre-miRNA-20b mimic and NC simulation into zebrafish embryos, embryo morphology at different time points. (B) Total RNA isolated from 96h post-injection embryos was used to determine FGF2 and GRB2 levels by real-time RT-PCR. The results showed that RNA expression was reduced compared to the control group.

The zebrafish model confirms the inhibitory effect of miR-20b. (A) Observation of retinal tissue sections at 96hpf. We found that in the miRNA-20b mimic group, there were fewer cells in the photoreceptor layer than in the control and wild groups, and the arrangement of cells was disordered. PRL: Photoreceptor layer, ONL: Outer nuclear layer, INL: Inner nuclear layer, GCL: Ganglion cell layer. (B, C) Immunofluorescence detected decreased FGF2 and GRB2 brightness compared to controls. Relative fluorescence values were analyzed using Imagej software (*P<0.05).