Ethanol decreases anxiety in the novel approach test. (a) Trackplot of an individual control zebrafish behaviour in the 5 minute trial. (b) Trackplot of an individual zebrafish exposed to 1.5% ethanol. (c) Heatmap (coloured representation of zebrafish location over the trial) for the same fish shown in (a). (d) Heatmap for the same fish shown in (b). (e) Time fish spent in the inner zone containing the novel object for different doses of ethanol. **P < 0.01, Kruskal-Wallis test with Dunn’s multiple comparison post hoc test.

Scopolamine decreases anxiety in the novel approach test. (a) Trackplot of an individual control zebrafish behaviour in the 5 minute trial. (b) Trackplot of an individual zebrafish exposed to 800 μM scopolamine. (c) Heatmap (coloured representation of zebrafish location over the trial) for the same fish shown in (a). (d) Heatmap for the same fish shown in (b). (e) Time fish spent in the inner zone containing the novel object for different doses of scopolamine. **P < 0.01, Kruskal-Wallis test with Dunn’s multiple comparison post hoc test.

Scopolamine decreases anxiety in the novel tank diving test. (a) Heatmap of an individual control zebrafish behaviour in the 10 minute trial. (b) Heatmap of an individual zebrafish exposed to 800 μM scopolamine. (c) Time fish spent in each zone for an 800 μM dose of scopolamine (red bars) compared to controls (black bars). *P < 0.05, unpaired t-test.

Scopolamine decreases anxiety in a test of group behaviour. (a) Trackplot of a group of 6 control zebrafish for the first 30 seconds of the trial. Each coloured line represents an individual fish. (b) Trackplot of a group of 6 zebrafish dosed with 800 μM scopolamine. (c) Scopolamine (800 μM) increased the inter-individual distance in groups of zebrafish. (d) Scopolamine (800 μM) increased the nearest neighbour distance in groups of zebrafish. *P < 0.05, ****P < 0.0001, unpaired t-test.