The antithrombotic effect of probe AP. Zebrafish treated with arachidonic acid for 1.5 h were used as thrombosis model. A) Effects of probe AP on heart RBCs in thrombotic zebrafish. The heart red blood cells were stained with o-dianisidine staining. The heart RBCs intensity of zebrafish increased following AP probe treatment (1.5 h) in the presence of arachidonic acid (80 µM). B) Quantification data for Figure A. C) Effect of AP probe on thrombus formation in the caudal vein under a dissecting stereomicroscope. Images from aspirin or AP probe treatment are shown from a representative caudal vein of zebrafish from each group. Thrombus formation was markedly reduced in zebrafish treated with AP probe (1.5 h) in the presence of arachidonic acid. D) The therapeutic efficacy of AP probe on thrombosis formation.