- Title
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HS1BP3 negatively regulates autophagy by modulation of phosphatidic acid levels
- Authors
- Holland, P., Knævelsrud, H., Søreng, K., Mathai, B.J., Lystad, A.H., Pankiv, S., Bjørndal, G.T., Schultz, S.W., Lobert, V.H., Chan, R.B., Zhou, B., Liestøl, K., Carlsson, S.R., Melia, T.J., Di Paolo, G., Simonsen, A.
- Source
- Full text @ Nat. Commun.
HS1BP3 regulates autophagy in zebrafish. (a) Representative confocal images of GFP-LC3 puncta (autophagosomes) in the trunk area of GFP-LC3 transgenic zebrafish embryos injected with control morpholino (C), Hs1bp3 translational-blocking morpholino (K), and the human Hs1bp3 mRNA coinjected with the morpholino (R) and imaged at 2 dpf with or without pre-treatment with chloroquine (10 μM) for 6 h. Scale bars, 10 μm. (b) GFP-LC3 puncta were counted in the trunk region (marked in d) of the transgenic zebrafish embryos at 2 dpf (mean±s.e.m., n=3). Total of 7–13 embryos were used for each condition per experiment. *P<0.05, **P<0.01, ***P<0.001, by Student’s t-test. (c) Representative immunoblotting of Hs1bp3 and Tubulin in whole lysates of zebrafish embryos at 2 dpf, treated with or without chloroquine for 6 h before harvest. (d) Representative light fluorescent microscopy images of whole embryos at 2 dpf. Scale bars, 300 μm. |