- Title
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Methylome analysis for spina bifida shows SOX18 hypomethylation as a risk factor with evidence for a complex (epi)genetic interplay to affect neural tube development.
- Authors
- Rochtus, A., Winand, R., Laenen, G., Vangeel, E., Izzi, B., Wittevrongel, C., Moreau, Y., Verpoorten, C., Jansen, K., Van Geet, C., Freson, K.
- Source
- Full text @ Clin Epigenetics
Phenotype analysis of gene overexpression in zebrafish embryos. Pax2a staining after microinjection of abat mRNA (a) and sox18 mRNA (b). Wild-type (WT) zebrafish show expression in the hindbrain, hindbrain-midbrain boundary, neural tube, mesoderm, optic stalk, otic vesicle, and pronephric duct. Spinal cord malformation is indicated with an arrow. c Phenotype analysis after pax2a staining at 24 hpf resulted in respectively 50 and 74 % embryos with an affected phenotype after abat and sox18 overexpression. Folic acid supplementation after gene overexpression did not significantly influence the phenotype. ***P value <0.001 EXPRESSION / LABELING:
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