FIGURE SUMMARY
Title

Pro-Angiogenic Effects of Chalcone Derivatives in Zebrafish Embryos in Vivo

Authors
Chen, Y.H., Chang, C.Y., Chang, C.F., Chen, P.C., Lee, Y.T., Chern, C.Y., Tsai, J.N.
Source
Full text @ Molecules
Fig. 3

Chalcone derivatives had no effect on the formation of angiogenic intersegmental vessels (ISVs). (A–D) Representative images of zebrafish trunk ISVs treated with 3 ppm of chalcone and its derivatives (in 0.12% DMSO) via exposure method I. All figures are lateral views with dorsal to the top and anterior to the left. The fluorescent vessels of embryos were observed at 36 hpf. Vehicle: 0.12% DMSO only.

Fig. 4

Chalcone derivatives caused pro-angiogenic effect on the zebrafish transgenic line Tg(fli1:egfp). (A–D) Representative images of fluorescent SIV basket of embryos treated with water containing 0.12% DMSO (vehicle) or chalcones derivatives (in 0.12% DMSO) via exposure method II. The fluorescent vessels of embryos were observed at 72 hpf; (E–H) Enlarged SIV regions in (A–E). The number of SIV was defined as the sum of the number of vessel within the SIV plus the number of angiogenic outgrowth sprouts (marked by asters); (I) Data are the mean ± S.D. of twenty embryos from three independent experiments (n = 60 in each group). A t-test with 0.05 of significance level, carried out by JMP statistical software (version 4.02), was used to assess the difference in the number of SIV between two groups. The result reported the number of SIV for all experiment groups are significantly different from vehicle group (a: p < 0.0001).

Fig. 5

Chalcone derivatives caused pro-angiogenic effect on the wild type zebrafish. (A–F) Representative images of AP-stained SIV basket of embryos treated with GS4012, HMC, and chalcone derivatives via exposure method II; (G–L) Enlarged SIV regions in (A–F). The number of SIV was defined as the sum of the number of vessel within the SIV plus the number of angiogenic outgrowth sprouts (marked by asters). Embryos at 72 hpf were subjected to alkaline phosphatase staining.

Fig. 6

Effects of chalcone derivatives on the expression of vegfaa165.Whole-mount in situ hybridization was utilized to analyze the expression of vegfaa165 in zebrafish embryos treated with DMSO vehicle (A); 3 ppm of compound 1a (B); 1c (C) or 1e (D) via exposure method I. Embryos at 36 hpf were subjected to whole-mount in situ hybridization. All figures were lateral views with anterior to the left and dorsal at the top.

Acknowledgments
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