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Fig. 4 Chalcone derivatives caused pro-angiogenic effect on the zebrafish transgenic line Tg(fli1:egfp). (A–D) Representative images of fluorescent SIV basket of embryos treated with water containing 0.12% DMSO (vehicle) or chalcones derivatives (in 0.12% DMSO) via exposure method II. The fluorescent vessels of embryos were observed at 72 hpf; (E–H) Enlarged SIV regions in (A–E). The number of SIV was defined as the sum of the number of vessel within the SIV plus the number of angiogenic outgrowth sprouts (marked by asters); (I) Data are the mean ± S.D. of twenty embryos from three independent experiments (n = 60 in each group). A t-test with 0.05 of significance level, carried out by JMP statistical software (version 4.02), was used to assess the difference in the number of SIV between two groups. The result reported the number of SIV for all experiment groups are significantly different from vehicle group (a: p < 0.0001).