FIGURE SUMMARY
Title

Nerve Growth Factor Stimulates Cardiac Regeneration via Cardiomyocyte Proliferation in Experimental Heart Failure

Authors
Lam, N.T., Currie, P.D., Lieschke, G.J., Rosenthal, N.A., and Kaye, D.M.
Source
Full text @ PLoS One
Fig. 1

Experimental heart failure model in zebrafish.

A Zebrafish (100 hpf) without HF (left panel) and with the HF phenotype (right panel). Bar graphs showing B. Ventricular fractional area change at 96 hpf, C. Incidence of AA induced HF at 168 hpf. D. Incidence of AA induced mortality at 168 hpf. ***p<0.001.
Fig. 2

Aristolochic acid increases apoptosis in the heart, deplete CMs and reduces CM proliferation.

Bar graphs demonstrating A. Expression of caspase 3 mRNA in the heart at 96 hpf, B. Time dependent changes cardiomyocyte number and C. Abundance of BrdU positive cardiomyocytes at 100 hpf.
Fig. 3

NGF decreases the incidence of AA induced HF and death.

Kaplan Meier Curves showing A. Effect of NGF on frequency of HF, B. Effect of NGF on frequency of death and C. Effect of the NGF trkA receptor antagonist, K-252a, on frequency of HF. D. Effect of K-252a on frequency of death. **p<0.01, ***p<0.001.
Fig. 4

NGF does not attenuate AA induced apoptosis.

A. Bar graph represents absence of effect of NGF on caspase 3 mRNA expression in AA treated (72–75 hpf) zebrafish hearts at 96 hpf. B and C. Representative IMARIS images of cardiomyocyte TUNEL staining in control and AA treated zebrafish heart at 100 hpf. Scale bar = 50 µm. D. Bar graph showing quantitative analysis of TUNEL staining (n = 6 per group, * p<0.05 vs control).
Fig. 5

NGF stimulates CM proliferation following AA exposure.

A. Bar graph showing the effect of NGF on cardiomyocyte proliferation. B–D. Representative IMARIS images of BrdU+ cardiomyocytes in the heart (76–100 hpf) from control (B), AA treated (C) and AA treated and post treated with NGF (D). Scale Bars = 50 µm. *p<0.05, **p<0.01.

EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Day 4
Fig. 6

NGF restores total CM number following AA exposure.

Bar graphs showing that NGF restores total cardiomyocyte number in zebrafish model of experimental heart failure. Time dependent effects at A. 96 hpf, B. 120 hpf, and C. 144 hpf. **p<0.01.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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