Expression pattern of pdzrn3. (A,B) Three-somite (A) and six-somite (B) stage: dorsal views, anterior to the left. (C-E) twelve-somite (C) and eighteen-somite (D) stage: lateral views, anterior to the left. (E) Eighteen-somite stage: dorsal view, anterior to the bottom. (F) Twenty-somite stage embryo stained for prolonged time: dorsal view, anterior to the left. (G,H) Comparison between the anterior expression of pax2.1a (G) and pdzrn3 (H) at 24-hpf stage: frontal views, dorsal to the top. (I,J) Comparison between the rhomboencephalic expressions of pdzrn3 (I, J; purple staining indicated by arrowheads), krox20 (I, turquoise staining) and pax2.1a (J, turquoise staining); dorsal view, anterior to the right. (K) 24-hpf stage embryo stained for prolonged time: lateral view, anterior to the left. The inset in the top right corner shows a magnification of somite expression in the trunk. (L) Head expression in 32 hpf embryo: ventral view anterior to the top; os, optic stalk; pt, posterior tuberculum; re, retina; th, thalamus. (M,N) 32 hpf stage: lateral view, anterior to the left; hm, head mesenchyme; pd, pronephric duct; pf, pectoral fin. To analyze internal brain expression sites the eyes of the embryo shown in N were removed. (O) Trunk and tail expression at 48 hpf: dorsal to the top, anterior to the left. Arrows point to the motor neurons of the spinal cord. The bracket delimits pdzrn3 expression in the ventral region of somites.

Retinal expression of pdzrn3 in selected zebrafish mutants. (A-H) Retinal expression of pdzrn3 at 24-hpf stage in wild type (A,B,E,F), smo (C,D) and noi mutants (G,H). (A,C,E,G) ventral view, anterior to the top. (B,D) Lateral view, anterior to the left (F,H) latero-anterior view, dorsal to the top. Arrows in (G,H) point to the lack of pdzrn3 expression in the ventral retina of noi mutants.