ERBB3_MCS6 drives reporter expression in vivo in a pattern similar to erbb3b and under the control of sox10. (A-D) Expression pattern of ERBB3_MCS driving eGFP in G1 transgenic 24-72hpf zebrafish embryos. Arrows indicate tissues where expression was noted in multiple founders. (E-H) eGFP expression pattern seen in multiple founders of SOXE-2m ERBB3_MCS6 transgenic fish. Although expression is similar to WT ERBB3_MCS6 at 24hpf, expression differs at later stages of development. (I-P) Results of morpholino knockdown of sox10 in ERBB3_MCS6 transgenic embryos. (I-L) Normal expression of ERBB3_MCS6 in uninjected transgenic embryos at 24-48hpf. (M-P) Loss of reporter expression in embryos injected with a Sox10 morpholino at 24hpf and appearance of disorganized GFP expressing NC cells at 48hpf. Abbreviations: cranial neural crest (CNC), migratory crest (MC), pre-migratory crest (PMC), dorsal root ganglia (DRG), posterior lateral line (PLL), Schwann cells (SC), oligodendrocytes (OD) and notochord (NC).

ERBB3_MCS1 and ERBB3_MCS4 drive reporter expression in vivo in a pattern similar to erbb3b. (A-J) Expression pattern of the indicated MCS driving eGFP in G1 transgenic 24-72hpf zebrafish embryos. Arrows indicate tissues where expression was noted in multiple founders. Abbreviations: mesencephalon (M), hindbrain (HB), olfactory bulb (OB), pharyngeal arches (PA), cranial ganglia, posterior lateral line ganglia (PLLg).

Range of eGFP phenotypes in ERBB3_MCS6 transgenic fish upon sox10 morpholino injection. (A-B) eGFP expression driven by ERBB3_MCS6 in uninjected fish at 24hpf. Expression is noted in cranial neural crest (CNC), premigratory NC (PMC) and migratory crest (MC) (C-D) Fewer eGFP positive CNC (C) and PMC cells seen in sox10 morpholino injected transgenic embryos, and significantly reduced numbers of MC (D).