- Title
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Sex determination in the Squalius alburnoides complex: an initial characterization of sex cascade elements in the context of a hybrid polyploid genome
- Authors
- Pala, I., Schartl, M., Thorsteinsdóttir, S., and Coelho, M.M.
- Source
- Full text @ PLoS One
Dmrt1: (a) S. pyrenaicus (PP genotype); (b) S. alburnoides (AA genotype) and (c) S. alburnoides (PA genotype) testis; (d) S. alburnoides (PA genotype) and S. alburnoides (PAA genotype) ovary with (e) antisense and (f) sense probes. Wt1: (g) S. pyrenaicus (PP genotype); (h) S. alburnoides (AA genotype) and (i) S. alburnoides (PA genotype) testis; (j) S. alburnoides (PA genotype) and (k) S. alburnoides (PAA genotype) ovary. Dax1: (l) S. alburnoides (AA genotype), (m) S. pyrenaicus (PP genotype) and (n) S. alburnoides (PAA genotype) ovary. Examples of areas with positive signal are indicated by black arrows; examples of negative cells are highlighted with grey arrowheads. Germ cells (GS), early perinuclear (P), cortical alveolar (CA) and vitellogenic (V) oocytes. Scale bar = 100 μm (a, b, e, f, g, h, k, l, m); scale bar = 200 μm (c, d, i, j, n). |
(a) S. alburnoides (AA genotype) testis; (b) S. alburnoides (PAA genotype) ovary and S. pyrenaicus (PP genotype) ovary with (c) antisense and (d) sense probes (positive signals in primary oocytes exemplified with black arrows; lower expression in early stage oocytes exemplified with grey arrows; cells in different maturation stages, not expressing the transcript are indicated by grey arrowheads). Germ cells (GS), early perinuclear (P), cortical alveolar (CA) and vitellogenic (V) oocytes. Scale bar = 100 μm (a, c, d); scale bar = 200 μm (b). |
Expression of dmrt1 during S. alburnoides development. (a) RT-PCR analysis of dmrt1 in embryos at 2, 6 and 12 days after hatching (dah); (b) Location of dmrt1 expression in an embryo section at 6dah in the presumptive location of the developing gonad (arrow). Spinal cord (SC); gut (G); yolk sac (Y). Scale bar = 100 μm. |
Expression of wt1 during S. alburnoides development. (a) RT-PCR analysis of wt1 in embryos at 2, 4, 6 and 14 days after hatching (dah); (b) Location of wt1 expression in embryo sections at 6dah and 12dah in the presumptive location of the developing gonad (arrow). Mesoderm (M); gut (G); yolk sac (Y). Scale bar = 100 μm. |
Absence of dax1 positive signals in embryo sections of S. alburnoides (AA genotype). In situ hybridisation at 6 (sense probe in grey) and 12 days after hatching (dah). Spinal cord (SC); gut (G); yolk sac (Y). Scale bar = 100 μm. |
RT-PCR analysis of figla in adult gonad tissue. S. pyrenaicus (PP) females; S. alburnoides females (PAA) and males (AA). Actin controls for the same samples are shown in the lower row. |