ZFIN ID: ZDB-LAB-990719-1
Becker Lab
PI/Director: Becker, Thomas S.
Rinkwitz, Silke
Contact Person: Becker, Thomas S.
Email: tsbecker@med.usyd.edu.au
URL: http://sydney.edu.au/bmri/research/neurobiology-genomics/index.php
Address: Developmental Neurobiology and Genomics Brain and Mind Research Institute University of Sydney 100 Mallet Street Camperdown, NSW 2050 Australia
Country: Australia
Phone: +61-2-93510997
Fax: +61-2-93510599
Line Designation: bi

Show first 50 of 293 genomic features

We use the zebrafish as a model for the evolution of vertebrate genomes and for genomics of human disease. For example, all vertebrates have a very similar complement of genes, yet come in very different shapes and sizes. How do vertebrate genomes achieve these differences using essentially the same genes? The answer, in large part, is that the regulation of key developmental genes differs in different vertebrates (these are genes with regulatory function that have been characterized through their roles in animal development through genetic screens in model organisms such as fruit flies, zebrafish and mice). Differences in gene regulation, in evolution, have been achieved by exposing these genes to new regulatory sequences that may have had other roles previously, for example as mobile DNA elements or viruses, or may have originated from other regions of the same genome. While the precise regulation of this select group of genes is necessary to build a vertebrate organism, subtle mis-regulation of these genes may result in human disease. For example, type 2 diabetes may result when insufficient numbers of insulin-producing cells are made, or when these cells produce insufficient quantities of insulin, processes that are regulated by developmental genes. Likewise, subtle alterations in the generation of neuronal numbers or –connections within the human brain may result in psychiatric disease. Our group has shown that genomic regions involved in the regulation of developmental genes are large, evolutionary conserved segments of chromosomes and often stretch over multiple genes. We now aim to resolve human diseases that have been mapped to these regions by testing human regulatory DNA in transgenic zebrafish to pinpoint which genes are involved in human neurological diseases, and how mis-regulation of these genes may result in the observed defects. The current focus of the group is on characterizing gene regulation in the vertebrate brain of chromosomal loci implicated in obesity, and several types of psychiatric disorders.

Ishibashi, Minaka Post-Doc Mechaly, Alejandro Post-Doc Stavert, Bethany Graduate Student
Svahn, Adam Graduate Student Zhao, Ting Graduate Student Manning, Liz Technical Staff
Hussein, Abbas

Paasila, P.J., Fok, S.Y.Y., Flores-Rodriguez, N., Sajjan, S., Svahn, A.J., Dennis, C.V., Holsinger, R.M.D., Kril, J.J., Becker, T.S., Banati, R.B., Sutherland, G.T., Graeber, M.B. (2021) Ground state depletion microscopy as a tool for studying microglia-synapse interactions. Journal of neuroscience research. 99(6):1515-1532
Erdemci-Tandogan, G., Clark, M.J., Amack, J.D., Manning, M.L. (2018) Tissue Flow Induces Cell Shape Changes During Organogenesis. Biophysical journal. 115(11):2259-2270
Acosta, J.R., Watchon, M., Yuan, K.C., Fifita, J., Svahn, A.J., Don, E.K., Blair, I.P., Nicholson, G.A., Cole, N.J., Goldsbury, C., Laird, A.S. (2018) Neuronal cell culture from transgenic zebrafish models of neurodegenerative disease. Biology Open. 7(10):
Formella, I., Svahn, A.J., Radford, R.A.W., Don, E.K., Cole, N.J., Hogan, A., Lee, A., Chung, R.S., Morsch, M. (2018) Real-time visualization of oxidative stress-mediated neurodegeneration of individual spinal motor neurons in vivo. Redox Biology. 19:226-234
Svahn, A.J., Don, E.K., Badrock, A.P., Cole, N.J., Graeber, M.B., Yerbury, J.J., Chung, R., Morsch, M. (2018) Nucleo-cytoplasmic transport of TDP-43 studied in real time: impaired microglia function leads to axonal spreading of TDP-43 in degenerating motor neurons. Acta Neuropathologica. 136(3):445-459
Zhu, P., Ma, Z., Guo, L., Zhang, W., Zhang, Q., Zhao, T., Jiang, K., Peng, J., Chen, J. (2017) Short body length phenotype is compensated by the upregulation of nidogen family members in a deleterious nid1a mutation of zebrafish. Journal of genetics and genomics = Yi chuan xue bao. 44(11):553-556
Watchon, M., Yuan, K.C., Mackovski, N., Svahn, A.J., Cole, N.J., Goldsbury, C., Rinkwitz, S., Becker, T.S., Nicholson, G.A., Laird, A.S. (2017) Calpain inhibition is protective in Machado-Joseph disease zebrafish due to induction of autophagy. The Journal of neuroscience : the official journal of the Society for Neuroscience. 37(32):7782-7794
Mechaly, A.S., Richardson, E., Rinkwitz, S. (2017) Activity of etv5a and etv5b genes in the hypothalamus of fasted zebrafish is influenced by serotonin. General and comparative endocrinology. 246:233-240
Cobbina, S.J., Mao, G., Zhao, T., Xu, H., Zhang, Z., Liu, H., Zou, Y., Wu, X., Yang, L. (2017) Modulation of N-Methyl-D-Aspartate Receptors (NMDAR), Bcl-2 and C-Fos Gene Expressions on Exposure to Individual and Mixtures of Low Concentration Metals in Zebrafish (Danio rerio). Archives of environmental contamination and toxicology. 72(3):418-427
Mu, X., Zhao, T., Xu, C., Shi, W., Geng, B., Shen, J., Zhang, C., Pan, J., Yang, J., Hu, S., Lv, Y., Wen, H., You, Q. (2017) Oncometabolite succinate promotes angiogenesis by upregulating VEGF expression through GPR91-mediated STAT3 and ERK activation. Oncotarget. 8(8):13174-13185
Soulika, M., Kaushik, A., Mathieu, B., Lourenço, R., Komisarczuk, A.Z., Romano, S.A., Jouary, A., Lardennois, A., Tissot, N., Okada, S., Abe, K., Becker, T.S., Kapsimali, M. (2016) Diversity in cell motility reveals the dynamic nature of the formation of zebrafish taste sensory organs. Development (Cambridge, England). 143(11):2012-24
Giacomotto, J., Carroll, A.P., Rinkwitz, S., Mowry, B., Cairns, M.J., Becker, T.S. (2016) Developmental suppression of schizophrenia-associated miR-137 alters sensorimotor function in zebrafish. Translational psychiatry. 6:e818
Laird, A.S., Mackovski, N., Rinkwitz, S., Becker, T.S., Giacomotto, J. (2016) Tissue-specific models of spinal muscular atrophy confirm a critical role of SMN in motor neurons from embryonic to adult stages. Human molecular genetics. 25(9):1728-38
Svahn, A.J., Giacomotto, J., Graeber, M.B., Rinkwitz, S., Becker, T.S. (2016) miR-124 contributes to the functional maturity of microglia. Developmental Neurobiology. 76(5):507-18
Abu-Siniyeh, A., Owen, D.M., Benzing, C., Rinkwitz, S., Becker, T.S., Majumdar, A., Gaus, K. (2016) The aPKC/Par3/Par6 polarity complex and membrane order are functionally inter-dependent in epithelia during vertebrate organogenesis. Traffic (Copenhagen, Denmark). 17(1):66-79
Cobbina, S.J., Xu, H., Zhao, T., Mao, G., Zhou, Z., Wu, X., Liu, H., Zou, Y., Wu, X., Yang, L. (2015) A multivariate assessment of innate immune-related gene expressions due to exposure to low concentration individual and mixtures of four kinds of heavy metals on zebrafish (Danio rerio) embryos. Fish & shellfish immunology. 47(2):1032-42
Ishibashi, M., Manning, E., Shoubridge, C., Krecsmarik, M., Hawkins, T.A., Giacomotto, J., Zhao, T., Mueller, T., Bader, P.I., Cheung, S.W., Stankiewicz, P., Bain, N.L., Hackett, A., Reddy, C.C., Mechaly, A.S., Peers, B., Wilson, S.W., Lenhard, B., Bally-Cuif, L., Gecz, J., Becker, T.S., Rinkwitz, S. (2015) Copy number variants in patients with intellectual disability affect the regulation of ARX transcription factor gene. Human genetics. 134(11-12):1163-82
Greenlees, R., Mihelec, M., Yousoof, S., Speidel, D., Wu, S.K., Rinkwitz, S., Prokudin, I., Perveen, R., Cheng, A., Ma, A., Nash, B., Gillespie, R., Loebel, D.A., Clayton-Smith, J., Lloyd, I.C., Grigg, J.R., Tam, P.P., Yap, A.S., Becker, T.S., Black, G.C., Semina, E., Jamieson, R.V. (2015) Mutations in SIPA1L3 cause eye defects through disruption of cell polarity and cytoskeleton organization. Human molecular genetics. 24(20):5789-804
Rinkwitz, S., Geng, F., Manning, E., Suster, M., Kawakami, K., Becker, T.S. (2015) BAC transgenic zebrafish reveal hypothalamic enhancer activity around obesity associated SNP rs9939609 within the human FTO gene. Genesis (New York, N.Y. : 2000). 53(10):640-51
Giacomotto, J., Rinkwitz, S., Becker, T.S. (2015) Effective heritable gene knockdown in zebrafish using synthetic microRNAs. Nature communications. 6:7378
Punnamoottil, B., Rinkwitz, S., Giacomotto, J., Svahn, A.J., Becker, T.S. (2015) Motor neuron-expressed microRNAs 218 and their enhancers are nested within introns of SLIT2/3 genes. Genesis (New York, N.Y. : 2000). 53(5):321-8
Naville, M., Ishibashi, M., Ferg, M., Bengani, H., Rinkwitz, S., Krecsmarik, M., Hawkins, T.A., Wilson, S.W., Manning, E., Chilamakuri, C.S., Wilson, D.I., Louis, A., Lucy Raymond, F., Rastegar, S., Strähle, U., Lenhard, B., Bally-Cuif, L., van Heyningen, V., FitzPatrick, D.R., Becker, T.S., Roest Crollius, H. (2015) Long-range evolutionary constraints reveal cis-regulatory interactions on the human X chromosome. Nature communications. 6:6904
Chang, G.H., Lay, A.J., Ting, K.K., Zhao, Y., Coleman, P.R., Powter, E.E., Formaz-Preston, A., Jolly, C.J., Bower, N.I., Hogan, B.M., Rinkwitz, S., Becker, T.S., Vadas, M.A., Gamble, J.R. (2014) ARHGAP18: an endogenous inhibitor of angiogenesis, limiting tip formation and stabilizing junctions. Small GTPases. 5(3):1-15
Giacomotto, J., Brouilly, N., Walter, L., Mariol, M.C., Berger, J., Ségalat, L., Becker, T.S., Currie, P.D., and Gieseler, K. (2013) Chemical genetics unveils a key role of mitochondrial dynamics, cytochrome c release and IP3R activity in muscular dystrophy. Human molecular genetics. 22(22):4562-78
Maley, D., Laird, A.S., Rinkwitz, S., and Becker, T.S. (2013) A Simple and Efficient Protocol for the Treatment of Zebrafish Colonies Infected with Parasitic Nematodes. Zebrafish. 10(3):447-50
Ishibashi, M., Mechaly, A.S., Becker, T.S., and Rinkwitz, S. (2013) Using zebrafish transgenesis to test human genomic sequences for specific enhancer activity. Methods (San Diego, Calif.). 62(3):216-25
Nocillado, J.N., Mechaly, A.S., and Elizur, A. (2013) In silico analysis of the regulatory region of the Yellowtail Kingfish and Zebrafish Kiss and Kiss receptor genes. Fish physiology and biochemistry. 39(1):59-63
Svahn, A.J., Graeber, M.B., Ellett, F., Lieschke, G.J., Rinkwitz, S., Bennett, M.R., and Becker, T.S. (2013) Development of ramified microglia from early macrophages in the zebrafish optic tectum. Developmental Neurobiology. 73(1):60-71
Djiotsa, J., Verbruggen, V., Giacomotto, J., Ishibashi, M., Manning, E., Rinkwitz, S., Manfroid, I., Lvoz, M., and Peers, B. (2012) Pax4 is not essential for beta-cell differentiation in zebrafish embryos but modulates alpha-cell generation by repressing arx gene expression. BMC Developmental Biology. 12(1):37
Folgueira, M., Bayley, P., Navratilova, P., Becker, T.S., Wilson, S.W., and Clarke, J.D. (2012) Morphogenesis underlying the development of the everted teleost telencephalon. Neural Development. 7(1):32
Coolen, M., Thieffry, D., Drivenes, O., Becker, T.S., and Bally-Cuif, L. (2012) miR-9 Controls the Timing of Neurogenesis through the Direct Inhibition of Antagonistic Factors. Developmental Cell. 22(5):1052-1064
Becker, T.S., and Rinkwitz, S. (2012) Zebrafish as a genomics model for human neurological and polygenic disorders. Developmental Neurobiology. 72(3):415-428
Sundström, E., Komisarczuk, A.Z., Jiang, L., Golovko, A., Navratilova, P., Rinkwitz, S., Becker, T.S., and Andersson, L. (2012) Identification of a melanocyte-specific, microphthalmia-associated transcription factor-dependent regulatory element in the intronic duplication causing hair greying and melanoma in horses. Pigment cell & melanoma research. 25(1):28-36
Rinkwitz, S., Mourrain, P., and Becker, T.S. (2011) Zebrafish: an integrative System for Neurogenomics and Neurosciences. Prog. Neurobiol.. 93(2):231-243
Labalette, C., Bouchoucha, Y.X., Wassef, M.A., Gongal, P.A., Le Men, J., Becker, T., Gilardi-Hebenstreit, P., and Charnay, P. (2011) Hindbrain patterning requires fine-tuning of early krox20 transcription by Sprouty 4. Development (Cambridge, England). 138(2):317-326
Punnamoottil, B., Herrmann, C., Pascual Anaya, J., D'Aniello, S., Garcia-Fernàndez, J., Akalin, A., Becker, T.S., and Rinkwitz, S. (2010) Cis-regulatory characterization of sequence conservation surrounding the Hox4 genes. Developmental Biology. 340(2):269-282
Dong, X., Navratilova, P., Fredman, D., Drivenes, Ø., Becker, T.S., and Lenhard, B. (2010) Exonic remnants of whole-genome duplication reveal cis-regulatory function of coding exons. Nucleic acids research. 38(4):1071-1085
Navratilova, P., Fredman, D., Lenhard, B., and Becker, T.S. (2010) Regulatory divergence of the duplicated chromosomal loci sox11a/b by subpartitioning and sequence evolution of enhancers in zebrafish. Molecular genetics and genomics : MGG. 283(2):171-184
Ragvin, A., Moro, E., Fredman, D., Navratilova, P., Drivenes, O., Engström, P.G., Alonso, M.E., Mustienes, E.D., Gomez Skarmeta, J.L., Tavares, M.J., Casares, F., Manzanares, M., van Heyningen, V., Molven, A., Njølstad, P.R., Argenton, F., Lenhard, B., and Becker, T.S. (2010) Long-range gene regulation links genomic type 2 diabetes and obesity risk regions to HHEX, SOX4, and IRX3. Proceedings of the National Academy of Sciences of the United States of America. 107(2):775-780
Komisarczuk, A.Z., Kawakami, K., and Becker, T.S. (2009) Cis-regulation and chromosomal rearrangement of the fgf8 locus after the Teleost/tetrapod split. Developmental Biology. 336(2):301-312
Navratilova, P., and Becker, T.S. (2009) Genomic regulatory blocks in vertebrates and implications in human disease. Briefings in functional genomics & proteomics. 8(4):333-342
Ma, L.H., Punnamoottil, B., Rinkwitz, S., and Baker, R. (2009) Mosaic hoxb4a neuronal pleiotropism in zebrafish caudal hindbrain. PLoS One. 4(6):e5944
Amsterdam, A., Lai, K., Komisarczuk, A.Z., Becker, T.S., Bronson, R.T., Hopkins, N., and Lees, J.A. (2009) Zebrafish Hagoromo Mutants Up-Regulate fgf8 Postembryonically and Develop Neuroblastoma. Molecular cancer research : MCR. 7(6):841-850
Navratilova, P., Fredman, D., Hawkins, T.A., Turner, K., Lenhard, B., and Becker, T.S. (2009) Systematic human/zebrafish comparative identification of cis-regulatory activity around vertebrate developmental transcription factor genes. Developmental Biology. 327(2):526-540
Topp, S., Stigloher, C., Komisarczuk, A.Z., Adolf, B., Becker, T.S., and Bally-Cuif, L. (2008) Fgf signaling in the zebrafish adult brain: Association of Fgf activity with ventricular zones but not cell proliferation. The Journal of comparative neurology. 510(4):422-439
Komisarczuk, A.Z., Topp, S., Stigloher, C., Kapsimali, M., Bally-Cuif, L., and Becker, T.S. (2008) Enhancer detection and developmental expression of zebrafish sprouty1, a member of the fgf8 synexpression group. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(9):2594-2603
Punnamoottil, B., Kikuta, H., Pezeron, G., Erceg, J., Becker, T.S., and Rinkwitz, S. (2008) Enhancer detection in zebrafish permits the identification of neuronal subtypes that express Hox4 paralogs. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(8):2195-2208
Bessa, J., Tavares, M.J., Santos, J., Kikuta, H., Laplante, M., Becker, T.S., Gómez-Skarmeta, J.L., and Casares, F. (2008) meis1 regulates cyclin D1 and c-myc expression, and controls the proliferation of the multipotent cells in the early developing zebrafish eye. Development (Cambridge, England). 135(5):799-803
Hoppmann, V., Wu, J.J., Søviknes, A.M., Helvik, J.V., and Becker, T.S. (2008) Expression of the eight AMPA receptor subunit genes in the developing central nervous system and sensory organs of zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(3):788-799
Pézeron, G., Mourrain, P., Courty, S., Ghislain, J., Becker, T.S., Rosa, F.M., and David, N.B. (2008) Live analysis of endodermal layer formation identifies random walk as a novel gastrulation movement. Current biology : CB. 18(4):276-281
Meijer, A.H., van der Sar, A.M., Cunha, C., Lamers, G.E., Laplante, M.A., Kikuta, H., Bitter, W., Becker, T.S., and Spaink, H.P. (2008) Identification and real-time imaging of a myc-expressing neutrophil population involved in inflammation and mycobacterial granuloma formation in zebrafish. Developmental and comparative immunology. 32(1):36-49
Weiner, A.M., Allende, M.L., Becker, T.S., and Calcaterra, N.B. (2007) CNBP mediates neural crest cell expansion by controlling cell proliferation and cell survival during rostral head development. Journal of cellular biochemistry. 102(6):1553-1570
Becker, T.S., and Lenhard, B. (2007) The random versus fragile breakage models of chromosome evolution: a matter of resolution. Molecular genetics and genomics : MGG. 278(5):487-491
Herpin, A., Lelong, C., Becker, T., Favrel, P., and Cunningham, C. (2007) A tolloid homologue from the Pacific oyster Crassostrea gigas. Gene expression patterns : GEP. 7(6):700-708
Kikuta, H., Laplante, M., Navratilova, P., Komisarczuk, A.Z., Engstrom, P.G., Fredman, D., Akalin, A., Caccamo, M., Sealy, I., Howe, K., Ghislain, J., Pezeron, G., Mourrain, P., Ellingsen, S., Oates, A.C., Thisse, C., Thisse, B., Foucher, I., Adolf, B., Geling, A., Lenhard, B., and Becker, T.S. (2007) Genomic regulatory blocks encompass multiple neighboring genes and maintain conserved synteny in vertebrates. Genome research. 17(5):545-555
Shankaran, S.S., Sieger, D., Schroter, C., Czepe, C., Pauly, M.C., Laplante, M.A., Becker, T.S., Oates, A.C., and Gajewski, M. (2007) Completing the set of h/E(spl) cyclic genes in zebrafish: her12 and her15 reveal novel modes of expression and contribute to the segmentation clock. Developmental Biology. 304(2):615-632
Rinkwitz, S., Laplante, M., Kikuta, H., and Becker, T.S. (2007) A large-scale zebrafish enhancer screen. ZFIN Direct Data Submission.
Kikuta, H., Fredman, D., Rinkwitz, S., Lenhard, B., and Becker, T.S. (2007) Retroviral enhancer detection insertions in zebrafish combined with comparative genomics reveal genomic regulatory blocks - a fundamental feature of vertebrate genomes. Genome biology. 8(1):S4
Pezeron, G., Anselme, I., Laplante, M., Ellingsen, S., Becker, T.S., Rosa, F.M., Charnay, P., Schneider-Maunoury, S., Mourrain, P., and Ghislain, J. (2006) Duplicate sfrp1 genes in zebrafish: sfrp1a is dynamically expressed in the developing central nervous system, gut and lateral line. Gene expression patterns : GEP. 6(8):835-842
Hadrys, T., Punnamoottil, B., Pieper, M., Kikuta, H., Pezeron, G., Becker, T.S., Prince, V., Baker, R., and Rinkwitz, S. (2006) Conserved co-regulation and promoter sharing of hoxb3a and hoxb4a in zebrafish. Developmental Biology. 297(1):26-43
Stigloher, C., Ninkovic, J., Laplante, M., Geling, A., Tannhauser, B., Topp, S., Kikuta, H., Becker, T.S., Houart, C., and Bally-Cuif, L. (2006) Segregation of telencephalic and eye-field identities inside the zebrafish forebrain territory is controlled by Rx3. Development (Cambridge, England). 133(15):2925-2935
Laplante, M., Kikuta, H., Konig, M., and Becker, T.S. (2006) Enhancer detection in the zebrafish using pseudotyped murine retroviruses. Methods (San Diego, Calif.). 39(3):189-198
Gomez-Skarmeta, J.L., Lenhard, B., and Becker, T.S. (2006) New technologies, new findings, and new concepts in the study of vertebrate cis-regulatory sequences. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(4):870-885
Amsterdam, A., and Becker, T.S. (2005) Transgenes as screening tools to probe and manipulate the zebrafish genome. Developmental Dynamics : an official publication of the American Association of Anatomists. 234(2):255-268
Ellingsen, S., Laplante, M.A., Konig, M., Kikuta, H., Furmanek, T., Hoivik, E.A., and Becker, T.S. (2005) Large-scale enhancer detection in the zebrafish genome. Development (Cambridge, England). 132(17):3799-3811
Hardison, A.L., Lichten, L., Banerjee-Basu, S., Becker, T.S., and Burgess, S.M. (2005) The zebrafish gene claudinj is essential for normal ear function and important for the formation of the otoliths. Mechanisms of Development. 122(7-8):949-958
Herpin, A., Lelong, C., Becker, T., Rosa, F., Favrel, P., and Cunningham, C. (2005) Structural and functional evidence for a singular repertoire of BMP receptor signal transducing proteins in the lophotrochozoan Crassostrea gigas suggests a shared ancestral BMP/activin pathway. The FEBS journal. 272(13):3424-3440
Herpin, A., Lelong, C., Becker, T., Rosa, F.M., Favrel, P., and Cunningham, C. (2005) Structural and functional evidences for a type 1 TGF-beta sensu stricto receptor in the lophotrochozoan Crassostrea gigas suggest conserved molecular mechanisms controlling mesodermal patterning across bilateria. Mechanisms of Development. 122(5):695-705
Hadrys, T., Prince, V., Hunter, M., Baker, R., and Rinkwitz, S. (2004) Comparative genomic analysis of vertebrate Hox3 and Hox4 genes. The Journal of experimental zoology. 302(2):147-164
Kawakami, K., Amsterdam, A., Shimoda, N., Becker, T., Mugg, J., Shima, A., and Hopkins, N. (2000) Proviral insertions in the zebrafish hagoromo gene, encoding an F-box/WD40-repeat protein, cause stripe pattern anomalies. Current biology : CB. 10(8):463-466
Becker, T.S., Burgess, S.M., Amsterdam, A.H., Allende, M.L., and Hopkins, N. (1998) not really finished is crucial for development of the zebrafish outer retina and encodes a transcription factor highly homologous to human Nuclear Respiratory Factor-1 and avian Initiation Binding Repressor. Development (Cambridge, England). 125:4369-4378
Amsterdam, A., Yoon, C., Allende, M., Becker, T., Kawakami, K., Burgess, S., Gaiano, N., and Hopkins, N. (1997) Retrovirus-mediated insertional mutagenesis in zebrafish and identification of a molecular marker for embryonic germ cells. Cold Spring Harbor symposia on quantitative biology. 62:437-450
Allende, M.L., Amsterdam, A., Becker, T., Kawakami, K., Gaiano, N., and Hopkins, N. (1996) Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development. Genes & Development. 10(24):3141-3155
Gaiano, N., Amsterdam, A., Kawakami, K., Allende, M., Becker, T., and Hopkins, N. (1996) Insertional mutagenesis and rapid cloning of essential genes in zebrafish. Nature. 383(6603):829-832