ZFIN ID: ZDB-LAB-110815-1
Wei Hu lab
PI/Director: Hu, Wei
Co-PI / Senior
Zhu, Zuoyan
Contact Person: Ji, Chen
Email: huwei@ihb.ac.cn
Address: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Science, No. 7 Donghu South Road, Wuhan, 430072, China
Country: China
Phone: 86-27-68780051
Fax: 86-27-68780123
Line Designation: ihb

Show all 30 genomic features

1. Fish reproductive biology.
2. Fish Genetic Engineering and ecological risk evaluation of transgenic fish.
3. Reprogramming mechanism of zebrafish somatic cloned embryos.

Binbin, Tao Research Staff Ji, Chen Research Staff

Li, Y., Li, X., Ye, D., Zhang, R., Liu, C., He, M., Wang, H., Hu, W., Sun, Y. (2024) Endogenous biosynthesis of docosahexaenoic acid (DHA) regulates fish oocyte maturation by promoting pregnenolone production. Zoological research. 45:176188176-188
Liang, F., Dong, Z., Ye, J., Hu, W., Bhandari, R.K., Mai, K., Wang, X. (2023) In vivo DNA methylation editing in zebrafish. Epigenetics. 18:21923262192326
Ye, D., Liu, T., Li, Y., Wang, Y., Hu, W., Zhu, Z., Sun, Y. (2023) Identification of fish spermatogenic cells through high-throughput immunofluorescence against testis with an antibody set. Frontiers in endocrinology. 14:10443181044318
Lin, X., Liu, F., Meng, K., Liu, H., Zhao, Y., Chen, Y., Hu, W., Luo, D. (2022) Comprehensive Transcriptome Analysis Reveals Sex-Specific Alternative Splicing Events in Zebrafish Gonads. Life (Basel, Switzerland). 12(9)
Sun, X., Tao, B., Wang, Y., Hu, W., Sun, Y. (2022) Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic Monopterus albus. International Journal of Molecular Sciences. 23(11)
Tao, B., Hu, H., Chen, J., Chen, L., Luo, D., Sun, Y., Ge, F., Zhu, Z., Trudeau, V.L., Hu, W. (2022) Sinhcaf-dependent histone deacetylation is essential for primordial germ cell specification. EMBO reports. 23(6):e54387
Song, Y., Hu, W., Ge, W. (2021) Establishment of transgenic zebrafish (Danio rerio) models expressing fluorescence proteins in the oocytes and somatic supporting cells. General and comparative endocrinology. 314:113907
Li, Y., Huang, R., Chen, L., Li, Y., Li, Y., Liao, L., He, L., Zhu, Z., Wang, Y. (2021) Characterization of SR-B2a and SR-B2b genes and their ability to promote GCRV infection in grass carp (Ctenopharyngodon idellus). Developmental and comparative immunology. 124:104202
Song, Y., Peng, W., Luo, J., Zhu, Z., Hu, W. (2021) Organization of the gonadotropin-inhibitory hormone (Lpxrfa) system in the brain of zebrafish (Danio rerio). General and comparative endocrinology. 304:113722
Zhou, T., Zhou, B., Zhao, Y., Li, Q., Song, G., Zhu, Z., Long, Y., Cui, Z. (2021) Development of a Mucus Gland Bioreactor in Loach Paramisgurnus dabryanus. International Journal of Molecular Sciences. 22(2):
Jia, S., Wu, X., Wu, Y., Cui, X., Tao, B., Zhu, Z., Hu, W. (2020) Multiple Developmental Defects in sox11a Mutant Zebrafish with Features of Coffin-Siris Syndrome. International journal of biological sciences. 16:3039-3049
Song, Y., Chen, J., Tao, B., Luo, D., Zhu, Z., Hu, W. (2020) Kisspeptin2 regulates hormone expression in female zebrafish (Danio rerio) pituitary. Molecular and Cellular Endocrinology. 513:110858
Zhang, F., Li, X., He, M., Ye, D., Xiong, F., Amin, G., Zhu, Z., Sun, Y. (2020) Efficient generation of zebrafish maternal-zygotic mutants through transplantation of ectopically induced and Cas9/gRNA targeted primordial germ cells. Journal of genetics and genomics = Yi chuan xue bao. 47(1):37-47
Liu, Z.H., Li, Y.W., Hu, W., Chen, Q.L., Shen, Y.J. (2020) Mechanisms involved in tributyltin-enhanced aggressive behaviors and fear responses in male zebrafish. Aquatic toxicology (Amsterdam, Netherlands). 220:105408
Feng, K., Cui, X., Song, Y., Tao, B., Chen, J., Wang, J., Liu, S., Sun, Y., Zhu, Z., Trudeau, V.L., Hu, W. (2019) Gnrh3 regulates PGC proliferation and sex differentiation in developing zebrafish. Endocrinology. 161(1):
Ye, D., Wang, X., Wei, C., He, M., Wang, H., Wang, Y., Zhu, Z., Sun, Y. (2019) Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b. PLoS Genetics. 15:e1008306
Zhang, Y., Li, Z., Hu, W., Liu, Z. (2019) A Mitochondrial-Targeting Near-Infrared Fluorescent Probe for Visualizing and Monitoring Viscosity in Live Cells and Tissues. Analytical chemistry. 91(15):10302-10309
Mehjabin, R., Xiong, L., Huang, R., Yang, C., Chen, G., He, L., Liao, L., Zhu, Z., Wang, Y. (2019) Full-Length Transcriptome Sequencing and the Discovery of New Transcripts in the Unfertilized Eggs of Zebrafish (Danio rerio). G3 (Bethesda). 9(6):1831-1838
Mehjabin, R., Chen, L., Huang, R., Zhu, D., Yang, C., Li, Y., Liao, L., He, L., Zhu, Z., Wang, Y. (2019) Expression and localization of grass carp pkc-θ (protein kinase C theta) gene after its activation. Fish & shellfish immunology. 87:788-795
Ye, D., Zhu, L., Zhang, Q., Xiong, F., Wang, H., Wang, X., He, M., Zhu, Z., Sun, Y. (2019) Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealed by Lifetime Labeling of Germline. Marine biotechnology (New York, N.Y.). 21(2):217-228
Chen, L., Huang, R., Zhu, D., Wang, Y., Mehjabin, R., Li, Y., Liao, L., He, L., Zhu, Z., Wang, Y. (2018) Cloning of six serpin genes and their responses to GCRV infection in grass carp (Ctenopharyngodon idella). Fish & shellfish immunology. 86:93-100
Tao, B., Hu, H., Mitchell, K., Chen, J., Jia, H., Zhu, Z., Trudeau, V.L., Hu, W. (2018) Secretogranin-II plays a critical role in zebrafish neurovascular modeling. Journal of molecular cell biology. 10(5):388-401
Hu, H., Tao, B., Chen, J., Zhu, Z., Hu, W. (2018) Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish (Danio rerio).. International journal of biological sciences. 14:78-86
Xia, H., Zhong, C., Wu, X., Chen, J., Tao, B., Xia, X., Shi, M., Zhu, Z., Trudeau, V.L., Hu, W. (2017) Mettl3 Mutation Disrupts Gamete Maturation and Reduces Fertility in Zebrafish.. Genetics. 208(2):729-743
Luo, K., Li, Y., Ai, K., Xia, L., Zhang, J., Hu, W., Gao, W., Guo, L., Qi, Z., Yuan, H., Qiaoqing, X. (2017) Bioinformatics and expression analysis of finTRIM genes in grass carp, Ctenopharyngodon idella. Fish & shellfish immunology. 66:217-223
Luo, K., Li, Y., Xia, L., Hu, W., Gao, W., Guo, L., Tian, G., Qi, Z., Yuan, H., Qiaoqing, X. (2017) Analysis of the expression patterns of the novel large multigene TRIM gene family (finTRIM) in zebrafish. Fish & shellfish immunology. 66:224-230
Zhai, B., Hu, W., Sun, J., Chi, S., Lei, Y., Zhang, F., Zhong, C., Liu, Z. (2017) A two-photon fluorescent probe for nitroreductase imaging in living cells, tissues and zebrafish under hypoxia conditions. The Analyst. 142(9):1545-1553
Song, Y., Tao, B., Chen, J., Jia, S., Zhu, Z., Trudeau, V.L., Hu, W. (2017) GABAergic Neurons and Their Modulatory Effects on GnRH3 in Zebrafish. Endocrinology. 158(4):874-886
Ai, K., Luo, K., Li, Y., Hu, W., Gao, W., Fang, L., Tian, G., Ruan, G., Xu, Q. (2017) Expression pattern analysis of IRF4 and its related genes revealed the functional differentiation of IRF4 paralogues in teleost. Fish & shellfish immunology. 60:59-64
Wang, H., He, L., Pei, Y., Chu, P., Huang, R., Li, Y., Liao, L., Zhu, Z., Wang, Y. (2016) Cloning and characterization of Bax1 and Bax2 genes of Ctenopharyngodon idellus and evaluation of transcript expression in response to grass carp reovirus infection. Fish physiology and biochemistry. 42(5):1369-82
Hu, W., Zeng, L., Wang, Y., Liu, Z., Ye, X., Li, C. (2016) A ratiometric two-photon fluorescent probe for fluoride ion imaging in living cells and zebrafish. The Analyst. 141(18):5450-5
Liu, L., Pan, L., Li, K., Zhang, Y., Zhu, Z., Sun, Y. (2016) Zebrafish Health Conditions in the China Zebrafish Resource Center and 20 Major Chinese Zebrafish Laboratories. Zebrafish. 13 Suppl 1:S8-S18
Fenghua, Z., Houpeng, W., Siyu, H., Feng, X., Zuoyan, Z., Yonghua, S. (2016) A comparison of the knockout efficiencies of two codon-optimized Cas9 coding sequences in zebrafish embryos. Yi chuan = Hereditas. 38:144-54
Shu, Y., Lou, Q., Dai, Z., Dai, X., He, J., Hu, W., Yin, Z. (2016) The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models. Scientific Reports. 6:18597
Pan, Y.X., Luo, Z., Zhuo, M.Q., Hu, W., Wu, K., Shi, X., Xu, Y.H. (2016) Liver X Receptor (LXR) in yellow catfish Pelteobagrus fulvidraco: Molecular characterization, mRNA tissue expression and transcriptional regulation by insulin in vivo and in vitro. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 191:13-9
He, M.D., Zhang, F.H., Wang, H.L., Wang, H.P., Zhu, Z.Y., Sun, Y.H. (2015) Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos. Mutation research. 780:86-96
Huang, R., Zhu, Z., Wang, Y. (2015) LG24 and sex chromosomes. Oncotarget. 6:26543
Jiang, Y., He, L., Ju, C., Pei, Y., Ji, M., Li, Y., Liao, L., Jang, S., Zhu, Z., Wang, Y. (2015) Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection. Fish & shellfish immunology. 44(1):88-99
Long, L., Guo, H., Yao, D., Xiong, K., Li, Y., Liu, P., Zhu, Z., Liu, D. (2015) Regulation of transcriptionally active genes via the catalytically inactive Cas9 in C. elegans and D. rerio. Cell Research. 25:638-41
Zeng, L., Chen, S., Xia, T., Hu, W., Li, C., Liu, Z. (2015) Two-Photon Fluorescent Probe for Detection of Exogenous and Endogenous Hydrogen Persulfide and Polysulfide in Living Organisms. Analytical chemistry. 87(5):3004-10
Chen, R., Qi, J., Yuan, H., Wu, Y., Hu, W., Xia, C. (2015) Crystal structures for short-chain pentraxin from zebrafish demonstrate a cyclic trimer with new recognition and effector faces. Journal of structural biology. 189(3):259-68
Song, Y., Duan, X., Chen, J., Huang, W., Zhu, Z., Hu, W. (2015) The distribution of Kiss1- and Kiss2-positive neurones and their connections with GnRH3 neurones in the zebrafish brain. Journal of neuroendocrinology. 27(3):198-211
Tang, H., Liu, Y., Luo, D., Ogawa, S., Yin, Y., Li, S., Zhang, Y., Hu, W., Parhar, I.S., Lin, H., Liu, X., Cheng, C.H. (2015) The kiss/kissr systems are dispensable for zebrafish reproduction: evidence from gene knockout studies. Endocrinology. 156(2):589-99
Zhang, Y., Chen, J., Cui, X., Luo, D., Xia, H., Dai, J., Zhu, Z., Hu, W. (2015) A controllable on-off strategy for the reproductive containment of fish. Scientific Reports. 5:7614
Qiu, C., Cheng, B., Zhang, Y., Huang, R., Liao, L., Li, Y., Luo, D., Hu, W., Wang, Y. (2014) Efficient Knockout of Transplanted Green Fluorescent Protein Gene in Medaka Using TALENs. Marine biotechnology (New York, N.Y.). 16(6):674-83
Chu, L., Li, J., Liu, Y., Hu, W., Cheng, C.H. (2014) Targeted gene disruption in zebrafish reveals noncanonical functions of Lh signaling in reproduction. Molecular endocrinology (Baltimore, Md.). 28(11):1785-95
Pang, S.C., Wang, H.P., Li, K.Y., Zhu, Z.Y., Kang, J.X., Sun, Y.H. (2014) Double Transgenesis of Humanized fat1 and fat2 Genes Promotes Omega-3 Polyunsaturated Fatty Acids Synthesis in a Zebrafish Model. Marine biotechnology (New York, N.Y.). 16(5):580-93
Liu, Y., Luo, D., Lei, Y., Hu, W., Zhao, H., Cheng, C.H. (2014) A highly effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish. Methods (San Diego, Calif.). 69(1):58-66
Wei, C.Y., Wang, H.P., Zhu, Z.Y., and Sun, Y.H. (2014) Transcriptional factors Smad1 and Smad9 act redundantly to mediate zebrafish ventral specification downstream of Smad5. The Journal of biological chemistry. 289(10):6604-18
Tong, X., Zu, Y., Li, Z., Li, W., Ying, L., Yang, J., Wang, X., He, S., Liu, D., Zhu, Z., Chen, J., Lin, S., and Zhang, B. (2014) Kctd10 regulates heart morphogenesis by repressing the transcriptional activity of Tbx5a in zebrafish. Nature communications. 5:3153
Zheng, J.L., Luo, Z., Zhu, Q.L., Tan, X.Y., Chen, Q.L., Sun, L.D., and Hu, W. (2013) Molecular cloning and expression pattern of 11 genes involved in lipid metabolism in yellow catfish Pelteobagrus fulvidraco. Gene. 531(1):53-63
Liu, Y., Luo, D., Zhao, H., Zhu, Z., Hu, W., and Cheng, C.H. (2013) Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes in Zebrafish Using TALENs. PLoS One. 8(10):e76387
Xiong, F., Wei, Z.Q., Zhu, Z.Y., and Sun, Y.H. (2013) Targeted Expression in Zebrafish Primordial Germ Cells by Cre/loxP and Gal4/UAS Systems. Marine biotechnology (New York, N.Y.). 15(5):526-39
Xia, Z., Tong, X., Liang, F., Zhang, Y., Kuok, C., Zhang, Y., Liu, X., Zhu, Z., Lin, S., and Zhang, B. (2013) Eif3ba regulates cranial neural crest development by modulating p53 in zebrafish. Developmental Biology. 381(1):83-96
Chen, J., Luo, Y.S., Zhu, Z.Y., and Hu, W. (2013) Cloning and promoter analysis of gonadotrophin-releasing hormone a in the orange-spotted grouper (Epinephelus coioides). Yi chuan = Hereditas. 35(7):896-902
Wang, Y.W., Wei, C.Y., Dai, H.P., Zhu, Z.Y., and Sun, Y.H. (2013) Subtractive phage display technology identifies zebrafish marcksb that is required for gastrulation. Gene. 521(1):69-77
Zu, Y., Tong, X., Wang, Z., Liu, D., Pan, R., Li, Z., Hu, Y., Luo, Z., Huang, P., Wu, Q., Zhu, Z., Zhang, B., and Lin, S. (2013) TALEN-mediated precise genome modification by homologous recombination in zebrafish. Nature Methods. 10(4):329-31
Huang, P., Zhu, Z., Lin, S., and Zhang, B. (2012) Reverse genetic approaches in zebrafish. Journal of genetics and genomics = Yi chuan xue bao. 39(9):421-433
Cao, M., Yang, Y., Xu, H., Duan, J., Cheng, N., Wang, J., Hu, W., and Zhao, H. (2012) Germ cell specific expression of Vasa in rare minnow, Gobiocypris rarus. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 162(3):163-170
Ishtiaq Ahmed, A.S., Xiong, F., Pang, S.C., He, M.D., Waters, M.J., Zhu, Z.Y., and Sun, Y.H. (2011) Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct. Transgenic Research. 20(3):557-567
Luo, D.J., Hu, W., Chen, S.P., and Zhu, Z.Y. (2011) Critical developmental stages for the efficiency of somatic cell nuclear transfer in zebrafish. International journal of biological sciences. 7(4):476-486
Guan, B., Ma, H., Wang, Y., Hu, Y., Lin, Z., Zhu, Z., and Hu, W. (2011) Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio). Marine biotechnology (New York, N.Y.). 13(2):336-44
Gao, W., Xu, L., Guan, R., Liu, X., Han, Y., Wu, Q., Xiao, Y., Qi, F., Zhu, Z., Lin, S., and Zhang, B. (2011) Wdr18 Is Required for Kupffer's Vesicle Formation and Regulation of Body Asymmetry in Zebrafish. PLoS One. 6(8):e23386
Dai, J., Cui, X., Zhu, Z., and Hu, W. (2010) Non-homologous end joining plays a key role in transgene concatemer formation in transgenic zebrafish embryos. International journal of biological sciences. 6(7):756-768
Song, W.H., Ding, F., Guo, J., Li, L.Y., Zhang, J.H., Lian, J., Hu, W.X., and Gao, M.L. (2010) Study on acute toxicity and structure-activity relationship of zebrafish (Danio rerio) exposed to naphthoquinones. Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes. 45(7):601-605
Xue, Y., Kuok, C., Xiao, A., Zhu, Z., Lin, S., and Zhang, B. (2010) Identification and expression analysis of mical family genes in zebrafish. Journal of genetics and genomics = Yi chuan xue bao. 37(10):685-693
Xiao, Z.G., Liu, H., Fu, J.P., Hu, W., Wang, Y.P., and Guo, Q.L. (2010) Cloning of common carp SOCS-3 gene and its expression during embryogenesis, GH-transgene and viral infection. Fish & shellfish immunology. 28(2):362-371
Chen, C.H., Sun, Y.H., Pei, D.S., and Zhu, Z.Y. (2009) Comparative expression of zebrafish lats1 and lats2 and their implication in gastrulation movements. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(11):2850-2859
Pei, D.S., Sun, Y.H., and Zhu, Z.Y. (2009) Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos. Molecular biology reports. 36(6):1375-1380
Li, X., He, J., Hu, W., and Yin, Z. (2009) The essential role of endogenous ghrelin in growth hormone expression during zebrafish adenohypophysis development. Endocrinology. 150(6):2767-2774
Luo, D., Hu, W., Chen, S., Xiao, Y., Sun, Y., and Zhu, Z. (2009) Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization. Biology of reproduction. 80(4):674-684
Ding, F., Song, W.H., Guo, J., Gao, M.L., and Hu, W.X. (2009) Oxidative stress and structure-activity relationship in the zebrafish (Danio rerio) under exposure to paclobutrazol. Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes. 44(1):44-50
Liu, J., Sun, Y.H., Wang, Y.W., Wang, N., Pei, D.S., Wang, Y.P., Hu, W., and Zhu, Z.Y. (2008) Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP. Theriogenology. 70(9):1525-1535
Pei, D.S., Sun, Y.H., Long, Y., and Zhu, Z.Y. (2008) Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique. Molecular biology reports. 35(2):139-143
Pei, D.S., Sun, Y.H., Chen, C.H., Chen, S.P., Wang, Y.P., Hu, W., and Zhu, Z.Y. (2008) Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos. BMC Developmental Biology. 8:29
Pei, D.S., Sun, Y.H., and Zhu, Z.Y. (2008) Construction of cytoplasmic molecular markers distinguishing Danio rerio from Gobiocypris rarus at high identity domains based on MP-PCR strategy and Sybr Green I detection. Molecular biology reports. 35(1):45-50
Pei, D.S., Sun, Y.H., Chen, S.P., Wang, Y.P., Hu, W., and Zhu, Z.Y. (2007) Identification of differentially expressed genes from the cross-subfamily cloned embryos derived from zebrafish nuclei and rare minnow enucleated eggs. Theriogenology. 68(9):1282-1291
Liu, W.Y., Wang, Y., Qin, Y., Wang, Y.P., and Zhu, Z.Y. (2007) Site-Directed Gene Integration in Transgenic Zebrafish Mediated by Cre Recombinase Using a Combination of Mutant Lox Sites. Marine biotechnology (New York, N.Y.). 9(4):420-428
Pei, D.S., Sun, Y.H., Chen, S.P., Wang, Y.P., Hu, W., and Zhu, Z.Y. (2007) Zebrafish GAPDH can be used as a reference gene for expression analysis in cross-subfamily cloned embryos. Analytical biochemistry. 363(2):291-293
Wu, Y., Zhang, G., Xiong, Q., Luo, F., Cui, C., Hu, W., Yu, Y., Su, J., Xu, A., and Zhu, Z. (2006) Integration of Double-Fluorescence Expression Vectors into Zebrafish Genome for the Selection of Site-Directed Knockout/Knockin. Marine biotechnology (New York, N.Y.). 8(3):304-311