ZFIN ID: ZDB-LAB-060314-2
Köster Lab
PI/Director: Köster, Reinhard W.
Contact Person: Köster, Reinhard W.
Email: r.koester@tu-bs.de
URL: https://www.tu-braunschweig.de/zoology/forschung/cellularmolecularneurobiology
Address: Technische Universität Braunschweig Institute of Zoology Division of Cellular and Molecular Neurobiology Spielmannstraße 7 38106 Braunschweig Germany
Country: Germany
Phone: +49-531-391-3230
Fax: +49-531-391-8178
Line Designation: bz

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We concentrate our research on the development, physiology and function of the zebrafish cerebellum. This crucial compartment of the vertebrate brain controls balance, body posture and locomotion - phenotypes that are easy to observe and analyze across vertebrates.

Pronounced neuronal migration is observed during cerebellar development making it a prime brain compartment to study cellular and molecular processes of neuronal cell motility. We try to elucidate on the cellular and subcellular level how migrating neuronal precursors initiate migration, interact with other cells, make pathfinding decisions, stop migration and adopt a specific fate.

Neurodegenerative diseases lead to severe neurological dysfunctions but their underlying cellular and molecular causes are poorly understood. We therefore aim to establish zebrafish disease models that are accessible for both in vivo monitoring of progressive neuronal degeneration and genetic modulation of disease-related processes for revealing entry sites to therapeutic treatment. As the development of the cerebellum, the underlying genetics, the histology, the neuronal connections and their physiology are highly conserved accross vertebrates, findings in zebrafish promise to hold true for mammals including humans.

Unlike mammals, zebrafish have an immense capacity to regenerate neuronal structures in any part of the central nervous system. In fact, the adult zebrafish cerebellum is characterized by pronounced neurogenesis and neuronal differentiation. This offers the unique chance to study neuronal regeneration processes in the vertebrate brain on the cellular and molecular level to identify mechanisms for triggering regeneration in the vertebrate brain.

Namikawa, Kazuhiko Post-Doc von Trotha, Jakob Post-Doc Russo, Giulio Graduate Student
Fritsch, Timo Fish Facility Staff Rascher, Monique Theisen, Ulrike

Pose-Méndez, S., Schramm, P., Valishetti, K., Köster, R.W. (2023) Development, circuitry, and function of the zebrafish cerebellum. Cellular and molecular life sciences : CMLS. 80:227227
Dorigo, A., Valishetti, K., Hetsch, F., Matsui, H., Meier, J.C., Namikawa, K., Köster, R.W. (2023) Functional regionalization of the differentiating cerebellar Purkinje cell population occurs in an activity-dependent manner. Frontiers in molecular neuroscience. 16:11669001166900
Pose-Méndez, S., Schramm, P., Winter, B., Meier, J.C., Ampatzis, K., Köster, R.W. (2023) Lifelong regeneration of cerebellar Purkinje cells after induced cell ablation in zebrafish. eLIFE. 12:
Lindemann, N., Kalix, L., Possiel, J., Stasch, R., Kusian, T., Köster, R.W., von Trotha, J.W. (2022) A comparative analysis of Danionella cerebrum and zebrafish (Danio rerio) larval locomotor activity in a light-dark test. Frontiers in behavioral neuroscience. 16:885775
Papadaki, S., Wang, X., Wang, Y., Zhang, H., Jia, S., Liu, S., Yang, M., Zhang, D., Jia, J.M., Köster, R.W., Namikawa, K., Piatkevich, K.D. (2022) Dual-expression system for blue fluorescent protein optimization. Scientific Reports. 12:10190
Russo, G., Unkauf, T., Meier, D., Wenzel, E.V., Langreder, N., Schneider, K.T., Wiesner, R., Bischoff, R., Stadler, V., Dübel, S. (2022) In vitro evolution of myc-tag antibodies: in-depth specificity and affinity analysis of Myc1-9E10 and Hyper-Myc. Biological chemistry. 403(5-6):479-494
Babakhanova, S., Jung, E.E., Namikawa, K., Zhang, H., Wang, Y., Subach, O.M., Korzhenevskiy, D.A., Rakitina, T.V., Xiao, X., Wang, W., Shi, J., Drobizhev, M., Park, D., Eisenhard, L., Tang, H., Köster, R.W., Subach, F.V., Boyden, E.S., Piatkevich, K.D. (2021) Rapid Directed Molecular Evolution of Fluorescent Proteins in Mammalian Cells. Protein science : a publication of the Protein Society. 31(3):728-751
Vauti, F., Vögele, V., Deppe, I., Hahnenstein, S.T., Köster, R.W. (2021) Structural Analysis and Spatiotemporal Expression of Atxn1 Genes in Zebrafish Embryos and Larvae. International Journal of Molecular Sciences. 22(21):
Chang, W., Pedroni, A., Köster, R.W., Giacomello, S., Ampatzis, K. (2021) Purkinje cells located in the adult zebrafish valvula cerebelli exhibit variable functional responses. Scientific Reports. 11:18408
Elsaey, M.A., Namikawa, K., Köster, R.W. (2021) Genetic Modeling of the Neurodegenerative Disease Spinocerebellar Ataxia Type 1 in Zebrafish. International Journal of Molecular Sciences. 22(14):
Buchberger, A., Schepergerdes, L., Flaßhoff, M., Kunick, C., Köster, R.W. (2021) A novel inhibitor rescues cerebellar defects in a zebrafish model of Down Syndrome-associated kinase Dyrk1A overexpression. The Journal of biological chemistry. 297(1):100853
Schramm, P., Hetsch, F., Meier, J.C., Köster, R.W. (2021) In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal. Journal of visualized experiments : JoVE. (168):
Theisen, U., Ernst, A.U., Heyne, R.L.S., Ring, T.P., Thorn-Seshold, O., Köster, R.W. (2020) Microtubules and motor proteins support zebrafish neuronal migration by directing cargo. The Journal of cell biology. 219(10):
Vauti, F., Stegemann, L.A., Vögele, V., Köster, R.W. (2020) All-age whole mount in situ hybridization to reveal larval and juvenile expression patterns in zebrafish. PLoS One. 15:e0237167
Chang, W., Pedroni, A., Hohendorf, V., Giacomello, S., Hibi, M., Köster, R.W., Ampatzis, K. (2020) Functionally distinct Purkinje cell types show temporal precision in encoding locomotion. Proceedings of the National Academy of Sciences of the United States of America. 117(29):17330-17337
Mattern, K., von Trotha, J.W., Erfle, P., Köster, R.W., Dietzel, A. (2020) NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish. Communications biology. 3:311
Li, J., Ünal, C.M., Namikawa, K., Steinert, M., Köster, R.W. (2020) Development of a Larval Zebrafish Infection Model for Clostridioides difficile. Journal of visualized experiments : JoVE. (156):
Namikawa, K., Dorigo, A., Köster, R.W. (2019) Neurological Disease Modelling for Spinocerebellar Ataxia Using Zebrafish. Journal of experimental neuroscience. 13:1179069519880515
Plate, J., Sassen, W.A., Hassan, A.H., Lehne, F., Köster, R.W., Kruse, T. (2019) S-Sulfocysteine Induces Seizure-Like Behaviors in Zebrafish. Frontiers in pharmacology. 10:122
Jagau, H., Behrens, I.K., Lahme, K., Lorz, G., Köster, R.W., Schneppenheim, R., Obser, T., Brehm, M.A., König, G., Kohler, T.P., Rohde, M., Frank, R., Tegge, W., Fulde, M., Hammerschmidt, S., Steinert, M., Bergmann, S. (2019) Von Willebrand Factor Mediates Pneumococcal Aggregation and Adhesion in Blood Flow. Frontiers in microbiology. 10:511
Namikawa, K., Dorigo, A., Zagrebelsky, M., Russo, G., Kirmann, T., Fahr, W., Dübel, S., Korte, M., Köster, R.W. (2019) Modeling neurodegenerative Spinocerebellar Ataxia type 13 in zebrafish using a Purkinje neuron specific tunable co-expression system. The Journal of neuroscience : the official journal of the Society for Neuroscience. 39(20):3948-3969
Fei, Z., Bae, K., Parent, S.E., Wan, H., Goodwin, K., Theisen, U., Tanentzapf, G., Bruce, A.E.E. (2018) A cargo model of yolk syncytial nuclear migration during zebrafish epiboly. Development (Cambridge, England). 146(1):
Russo, G., Lehne, F., Pose Méndez, S.M., Dübel, S., Köster, R.W., Sassen, W.A. (2018) Culture and Transfection of Zebrafish Primary Cells. Journal of visualized experiments : JoVE. (138):
Theisen, U., Hey, S., Hennig, C.D., Schnabel, R., Köster, R.W. (2018) Glycine is able to induce both a motility speed in- and decrease during zebrafish neuronal migration. Communicative & integrative biology. 11:1-7
Theisen, U., Hennig, C., Ring, T., Schnabel, R., Köster, R.W. (2018) Neurotransmitter-mediated activity spatially controls neuronal migration in the zebrafish cerebellum. PLoS Biology. 16:e2002226
Russo, G., Theisen, U., Fahr, W., Helmsing, S., Hust, M., Köster, R.W., Dübel, S. (2017) Sequence defined antibodies improve the detection of Cadherin 2 (N-Cadherin) during Zebrafish development. New biotechnology. 45:98-112
Sassen, W.A., Lehne, F., Russo, G., Wargenau, S., Dübel, S., Köster, R.W. (2017) Embryonic zebrafish primary cell culture for transfection and live cellular and subcellular imaging. Developmental Biology. 430(1):18-31
Weber, T., Namikawa, K., Winter, B., Müller-Brown, K., Kühn, R., Wurst, W., Köster, R.W. (2016) Caspase-mediated apoptosis induction in zebrafish cerebellar Purkinje neurons. Development (Cambridge, England). 143(22):4279-4287
Biechl, D., Dorigo, A., Köster, R.W., Grothe, B., Wullimann, M.F. (2016) Eppur Si Muove: Evidence for an External Granular Layer and Possibly Transit Amplification in the Teleostean Cerebellum. Frontiers in Neuroanatomy. 10:49
Matsui, H., Dorigo, A., Buchberger, A., Hocking, J.C., Distel, M., Köster, R.W. (2015) A zebrafish jam-b2 Gal4-enhancer trap line recapitulates endogenous jam-b2 expression in extraocular muscles. Developmental Dynamics : an official publication of the American Association of Anatomists. 244(12):1574-80
Matsui, H., Namikawa, K., Köster, R.W. (2014) Identification of the zebrafish red nucleus using Wheat Germ Agglutinin transneuronal tracing. Communicative & integrative biology. 7:e994383
von Trotha, J.W., Vernier, P., Bally-Cuif, L. (2014) Emotions and motivated behavior converge on an amygdala-like structure in the zebrafish. The European journal of neuroscience. 40(9):3302-3315
Schoetz, U., Deliolanis, N.C., Ng, D., Pauli, J., Resch-Genger, U., Kühn, E., Heuer, S., Beisker, W., Köster, R.W., Zitzelsberger, H., Caldwell, R.B. (2014) Usefulness of a Darwinian System in a Biotechnological Application: Evolution of Optical Window Fluorescent Protein Variants under Selective Pressure. PLoS One. 9:e107069
Matsui, H., Namikawa, K., Babaryka, A., Köster, R.W. (2014) Functional regionalization of the teleost cerebellum analyzed in vivo. Proceedings of the National Academy of Sciences of the United States of America. 111(32):11846-51
Flinn, L.J., Keatinge, M., Bretaud, S., Mortiboys, H., Matsui, H., De Felice, E., Woodroof, H.I., Brown, L., McTighe, A., Soellner, R., Allen, C.E., Heath, P.R., Milo, M., Muqit, M.M., Reichert, A.S., Köster, R.W., Ingham, P.W., and Bandmann, O. (2013) TigarB causes mitochondrial dysfunction and neuronal loss in PINK1 deficiency. Annals of neurology. 74(6):837-47
Weber, T., and Köster, R. (2013) Genetic tools for multicolor imaging in zebrafish larvae. Methods (San Diego, Calif.). 62(3):279-91
Hocking, J.C., Distel, M., and Köster, R.W. (2013) Studying cellular and subcellular dynamics in the developing zebrafish nervous system. Experimental neurology. 242:1-10
Xie, X., Mathias, J.R., Smith, M.A., Walker, S.L., Teng, Y., Distel, M., Koster, R.W., Sirotkin, H.I., Saxena, M.T., and Mumm, J.S. (2012) Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression in a NRSF/REST-dependent manner. BMC Biology. 10:93
Sasmal, P.K., Carregal-Romero, S., Han, A.A., Streu, C.N., Lin, Z., Namikawa, K., Elliott, S.L., Köster, R.W., Parak, W.J., and Meggers, E. (2012) Catalytic Azide Reduction in Biological Environments. Chembiochem : a European journal of chemical biology. 13(8):1116-1120
Oberoi, T.K., Dogan, T., Hocking, J.C., Scholz, R.P., Mooz, J., Anderson, C.L., Karreman, C., Heringdorf, D., Schmidt, G., Ruonala, M., Namikawa, K., Harms, G.S., Carpy, A., Macek, B., Köster, R.W., and Rajalingam, K. (2012) IAPs regulate the plasticity of cell migration by directly targeting Rac1 for degradation. The EMBO journal. 31(1):14-28
Walker, S.L., Ariga, J., Mathias, J.R., Coothankandaswamy, V., Xie, X., Distel, M., Köster, R.W., Parsons, M.J., Bhalla, K.N., Saxena, M.T., and Mumm, J.S. (2012) Automated reporter quantification in vivo: high-throughput screening method for reporter-based assays in zebrafish. PLoS One. 7(1):e29916
Hoegler, K.J., Distel, M., Köster, R.W., and Horne, J.H. (2011) Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines. Journal of visualized experiments : JoVE. (54)
Distel, M., Jennifer, C.H., and Köster, R.W. (2011) In vivo cell biology using Gal4-mediated multicolor subcellular labeling in zebrafish. Communicative & integrative biology. 4(3):336-9
Wullimann, M.F., Mueller, T., Distel, M., Babaryka, A., Grothe, B., and Köster, R.W. (2011) The long adventurous journey of rhombic lip cells in jawed vertebrates: a comparative developmental analysis. Frontiers in Neuroanatomy. 5:27
Santoriello, C., Gennaro, E., Anelli, V., Distel, M., Kelly, A., Köster, R.W., Hurlstone, A., and Mione, M. (2010) Kita Driven Expression of Oncogenic HRAS Leads to Early Onset and Highly Penetrant Melanoma in Zebrafish. PLoS One. 5(12):e15170
Distel, M., Hocking, J.C., Volkmann, K., and Köster, R.W. (2010) The centrosome neither persistently leads migration nor determines the site of axonogenesis in migrating neurons in vivo. The Journal of cell biology. 191(4):875-890
Volkmann, K., Chen, Y.Y., Harris, M.P., Wullimann, M.F., and Köster, R.W. (2010) The zebrafish cerebellar upper rhombic lip generates tegmental hindbrain nuclei by long-distance migration in an evolutionary conserved manner. The Journal of comparative neurology. 518(14):2794-2817
Kühn, E., and Köster, R.W. (2010) Analysis of gene expression by in situ hybridization on adult zebrafish brain sections. CSH protocols. 2010(2):pdb.prot5382
Anelli, V., Santoriello, C., Distel, M., Köster, R.W., Ciccarelli, F.D., and Mione, M. (2009) Global repression of cancer gene expression in a zebrafish model of melanoma is linked to epigenetic regulation. Zebrafish. 6(4):417-424
Rieger, S., Senghaas, N., Walch, A., and Köster, R.W. (2009) Cadherin-2 controls directional chain migration of cerebellar granule neurons. PLoS Biology. 7(11):e1000240
Distel, M., Wullimann, M.F., and Köster, R.W. (2009) Optimized Gal4 genetics for permanent gene expression mapping in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 106(32):13365-13370
Nikolaou, N., Watanabe-Asaka, T., Gerety, S., Distel, M., Köster, R.W., and Wilkinson, D.G. (2009) Lunatic fringe promotes the lateral inhibition of neurogenesis. Development (Cambridge, England). 136(15):2523-2533
Flinn, L., Mortiboys, H., Volkmann, K., Köster, R.W., Ingham, P.W., and Bandmann, O. (2009) Complex I deficiency and dopaminergic neuronal cell loss in parkin-deficient zebrafish (Danio rerio). Brain : a journal of neurology. 132(Pt 6):1613-1623
Senghaas, N., and Köster, R.W. (2009) Culturing and Transfecting Zebrafish PAC2 Fibroblast Cells. CSH protocols. 2009(6):pdb.prot5235
Paquet, D., Bhat, R., Sydow, A., Mandelkow, E.M., Berg, S., Hellberg, S., Fälting, J., Distel, M., Köster, R.W., Schmid, B., and Haass, C. (2009) A zebrafish model of tauopathy allows in vivo imaging of neuronal cell death and drug evaluation. J. Clin. Invest.. 119(5):1382-1395
Babaryka, A., Kuhn, E., and Koster, R.W. (2009) In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio. Journal of Fish Biology. 74(2):452-457
Chu, Y., Senghaas, N., Köster, R.W., Wurst, W., and Kühn, R. (2008) Novel caspase-suicide proteins for tamoxifen-inducible apoptosis. Genesis (New York, N.Y. : 2000). 46(10):530-536
Rieger, S., Volkmann, K., and Köster, R.W. (2008) Polysialyltransferase expression is linked to neuronal migration in the developing and adult zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(1):276-285
Volkmann, K., Rieger, S., Babaryka, A., and Köster, R.W. (2008) The zebrafish cerebellar rhombic lip is spatially patterned in producing granule cell populations of different functional compartments. Developmental Biology. 313(1):167-180
Koster, R.W., and Fraser, S.E. (2006) FGF signaling mediates regeneration of the differentiating cerebellum through repatterning of the anterior hindbrain and reinitiation of neuronal migration. The Journal of neuroscience : the official journal of the Society for Neuroscience. 26(27):7293-7304
Distel, M., Babaryka, A., Köster, R.W. (2006) Multicolor in vivo time-lapse imaging at cellular resolution by stereomicroscopy. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(3):843-845
von Trotha, J.W., Campos-Ortega, J.A., and Reugels, A.M. (2006) Apical localization of ASIP/PAR-3:EGFP in zebrafish neuroepithelial cells involves the oligomerization domain CR1, the PDZ domains, and the C-terminal portion of the protein. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(4):967-977
Rieger, S., Kulkarni, R.P., Darcy, D., Fraser, S.E., and Köster, R.W. (2005) Quantum dots are powerful multipurpose vital labeling agents in zebrafish embryos. Developmental Dynamics : an official publication of the American Association of Anatomists. 234(3):670-681
Köster, R.W., and Fraser, S.E. (2004) Time-lapse microscopy of brain development. The Zebrafish: Cellular and Developmental Biology,2nd Ed. Methods Cell Biol.. 76:207-235
Hove, J.R., Köster, R.W., Forouhar, A.S., Acevedo-Bolton, G., Fraser, S.E., and Gharib, M. (2003) Intracardiac fluid forces are an essential epigenetic factor for embryonic cardiogenesis. Nature. 421(6919):172-177
Wagner, T.U., Renn, J., Riemensperger, T., Volff, J.N., Köster, R.W., Goerlich, R., Schartl, M., and Winkler, C. (2003) The teleost fish medaka (Oryzias latipes) as genetic model to study gravity dependent bone homeostasis in vivo. Advances in space research : the official journal of the Committee on Space Research (COSPAR). 32(8):1459-1465
Köster, R.W. and Fraser, S.E. (2001) Direct imaging of in vivo neuronal migration in the developing cerebellum. Current biology : CB. 11(23):1858-1863
Köster, R.W. and Fraser, S.E. (2001) Tracing transgene expression in living zebrafish embryos. Developmental Biology. 233(2):329-346
Udvadia, A.J., Köster, R.W., and Skene, J.H. (2001) GAP-43 promoter elements in transgenic zebrafish reveal a difference in signals for axon growth during CNS development and regeneration. Development (Cambridge, England). 128(7):1175-1182
Götz, R., Köster, R.W., Winkler, C., Raulf, F., Lottspeich, F., Schartl, M., and Thoenen, H. (1994) Neurotrophin-6 is a new member of the nerve growth factor family. Nature. 372(6503):266-269