Mapping Details

GENETIC MAPPING PANELS

Chr	Location	Mapped As	Panel	Mapped By	Scoring
5	35.1 cM	z7351	Boston MGH Cross (MGH)	Fishman, Mark C.	Data
5	967.0 cR	z7351	Goodfellow T51 (T51)	Geisler, Robert	Data
5	23.2 cM	z7351	Heat Shock (HS)	Woods, Ian G.	Data
Note: Physical map location as displayed in the genome browsers is more precise than linkage map location. Note: Physical map location should be used whenever possible.

MAPPING FROM PUBLICATIONS

Marker	Type	Chr	Distance	Publication / Person	Comments
fc15g12	EST	5		Miller et al., 2004	Miller et al. (2004, Development. 131(10):2443-2461) mapped myst3 to LG 5 using bulk segregant analysis.
kat6a	GENE	5		Miller et al., 2004	Miller et al. (2004, Development. 131(10):2443-2461) mapped myst3 to LG 5 using bulk segregant analysis.
fc32e05	EST	5		Miller et al., 2004	Miller et al. (2004, Development. 131(10):2443-2461) mapped myst3 to LG 5 using bulk segregant analysis.
z6371	SSLP	5		Miller et al., 2004	Miller et al. (2004, Development. 131(10):2443-2461) mapped myst3 to LG 5 using bulk segregant analysis.
tbx1	GENE	5		Piotrowski et al., 2003	Piotrowski et al. (2003) used radiation hybrid and meiotic mapping panels to show that tbx1 and vgom208 lie on LG 5 between markers z7351/fb38h03 and z10456.
tm208	Feature	5		Piotrowski et al., 2003	Piotrowski et al. (2003) used radiation hybrid and meiotic mapping panels to show that tbx1 and vgom208 lie on LG 5 between markers z7351/fb38h03 and z10456.
z10456	SSLP	5		Piotrowski et al., 2003	Piotrowski et al. (2003) used radiation hybrid and meiotic mapping panels to show that tbx1 and vgom208 lie on LG 5 between markers z7351/fb38h03 and z10456.
fb38h03	EST	5		Piotrowski et al., 2003	Piotrowski et al. (2003) used radiation hybrid and meiotic mapping panels to show that tbx1 and vgom208 lie on LG 5 between markers z7351/fb38h03 and z10456.
vu166	Feature	5		Simmons et al., 2012	Simmons, et al. (2012. PLoS One 7(2):e32317.) reports mapping mutant locus  ... Simmons, et al. (2012. PLoS One 7(2):e32317.) reports mapping mutant locus vu166 to the pes gene on Chr5 between markers z6727 and z7351.
z6727	SSLP	5		Simmons et al., 2012	Simmons, et al. (2012. PLoS One 7(2):e32317.) reports mapping mutant locus  ... Simmons, et al. (2012. PLoS One 7(2):e32317.) reports mapping mutant locus vu166 to the pes gene on Chr5 between markers z6727 and z7351.
pes	GENE	5		Simmons et al., 2012	Simmons, et al. (2012. PLoS One 7(2):e32317.) reports mapping mutant locus  ... Simmons, et al. (2012. PLoS One 7(2):e32317.) reports mapping mutant locus vu166 to the pes gene on Chr5 between markers z6727 and z7351.

OTHER MAPPING INFORMATION

Chr 5 Miller et al., 2004 Miller et al. (2004, Development. 131(10):2443-2461) mapped myst3 to LG 5 using bulk  ... Miller et al. (2004, Development. 131(10):2443-2461) mapped myst3 to LG 5 using bulk segregant analysis.   Chr 5 Simmons et al., 2012 Simmons, et al. (2012. PLoS One 7(2):e32317.) reports mapping mutant locus vu166 to the pes gene on  ... Simmons, et al. (2012. PLoS One 7(2):e32317.) reports mapping mutant locus vu166 to the pes gene on Chr5 between markers z6727 and z7351.

Primer Sets:

Strain	Bandsize	Restriction Enzyme	Annealing Temperature [C]
IND	249,235		60.0
Forward Primer	CTCCGGTCCAGTTTGCTTTA
Reverse Primer	TCGTTTTGACATCAAGGTGC
AB	0,225		60.0
Forward Primer	CTCCGGTCCAGTTTGCTTTA
Reverse Primer	TCGTTTTGACATCAAGGTGC
TU	0,225		60.0
Forward Primer	CTCCGGTCCAGTTTGCTTTA
Reverse Primer	TCGTTTTGACATCAAGGTGC
EKW	0,245,225		60.0
Forward Primer	CTCCGGTCCAGTTTGCTTTA
Reverse Primer	TCGTTTTGACATCAAGGTGC