ZFIN ID: ZDB-GENE-980526-27

Mapping Details

Gene Name:	orthodenticle homeobox 2a
Symbol:	otx2a

PHYSICAL MAP AND BROWSER

Genome Browser	Chr	Position	Assembly
ZFIN	1	54,010,743 - 54,249,625	GRCz12tu
NCBI Map Viewer	1	54,010,743 - 54,249,625	GRCz12tu
Ensembl	1	50,921,266 - 50,929,957	GRCz11
NCBI Map Viewer	1	50,921,266 - 50,929,957	GRCz11
UCSC	1	Based on NM_131215	GRCz11
Vega	1	50,277,467 - 50,286,158	GRCv10

Mapped Clones containing otx2a
DKEYP-123H10	Chr: 1 Details

PHYSICAL MAPPING

Feature	Chr	Position	Assembly	Source	Citations
sa12472	1	54,247,359	GRCz12tu	DIRECT	Sealy et al., 2025
	1	50,928,849	GRCz11	DIRECT	Busch-Nentwich et al., 2013
	1	50,285,050	GRCz10	DIRECT	Busch-Nentwich et al., 2013
	1	51,438,171	Zv9	DIRECT	Busch-Nentwich et al., 2013
sa19590	1	54,242,715	GRCz12tu	DIRECT	Sealy et al., 2025
	1	50,924,205	GRCz11	DIRECT	Busch-Nentwich et al., 2013
	1	50,280,406	GRCz10	DIRECT	Busch-Nentwich et al., 2013
	1	51,433,527	Zv9	DIRECT	Busch-Nentwich et al., 2013
zko985a	1		GRCz11	OTHER_MAPPING	ZFIN Curated Data
zko985b	1		GRCz11	OTHER_MAPPING	ZFIN Curated Data

GENETIC MAPPING PANELS
Chr	Location	Mapped As	Panel	Mapped By	Scoring
1	152.6 cM	otx3	Mother of Pearl (MOP)	Postlethwait, John H.	Data
1	453.38 cR	otx3	Loeb/NIH/5000/4000 (LN54)	Dawid, Igor B.	Data
1	6389.0 cR	otx3	Goodfellow T51 (T51)	Geisler, Robert	Data
1	82.5 cM	otx3	Heat Shock (HS)	Woods, Ian G.	Data

Note: Physical map location as displayed in the genome browsers is more precise than linkage map location. Note: Physical map location should be used whenever possible.

Primer Sets:
Strain	Bandsize	Restriction Enzyme	Annealing Temperature [C]
SJD	450		36.0
Forward Primer	ACCACTGGCGTCCCGTCG
Reverse Primer	CCGCCGCAGAGCTGAAGT

Genomic Feature sa12472 is an allele of otx2a