Fig. 2 Mapk7-morpholino knockdown zebrafish embryos exhibited body curvature. (A) Schematic diagram of the targeting site of mapk7-MO. (B) RT-PCR analysis of the mapk7 MO-injected embryos showed that exon 5 of mapk7 was skipped. (C and D) Body curvature was observed in the morphants, and co-injection of human WT MAPK7 mRNA significantly reversed the phenotype. Scale Bar: 500 μm. N≥100. (E and F) The number of ossified vertebrae in the morphants decreased significantly in response to the mapk7-morpholinos. ***P< 0.01. PHENOTYPE:
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Fig. 4 Overexpression of rps6ka3b mRNA reversed the body curvature of mapk7 morphants. (A) Diagram demonstrating the targeting sites of rps6ka3-morpholinos. (B) RT-PCR analysis of the rps6ka3 MO-injected embryos was conducted to ensure knockdown efficiency. (C) Body curvature was observed in the morphants. (D) Percent distribution of normal embryos vs. embryos with axial deformity, or dead embryos. N≥100. PHENOTYPE:
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