FIGURE SUMMARY
Title

Mutant MAPK7-Induced Idiopathic Scoliosis is Linked to Impaired Osteogenesis

Authors
Zhou, T., Chen, C., Xu, C., Zhou, H., Gao, B., Su, D., Liao, Z., Li, Y., Yang, S., Su, P.
Source
Full text @ Cell Physiol. Biochem.
Fig. 2

Mapk7-morpholino knockdown zebrafish embryos exhibited body curvature. (A) Schematic diagram of the targeting site of mapk7-MO. (B) RT-PCR analysis of the mapk7 MO-injected embryos showed that exon 5 of mapk7 was skipped. (C and D) Body curvature was observed in the morphants, and co-injection of human WT MAPK7 mRNA significantly reversed the phenotype. Scale Bar: 500 ?m. N?100. (E and F) The number of ossified vertebrae in the morphants decreased significantly in response to the mapk7-morpholinos. ***P< 0.01.

PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Day 4 to Days 14-20
Fig. 4

Overexpression of rps6ka3b mRNA reversed the body curvature of mapk7 morphants. (A) Diagram demonstrating the targeting sites of rps6ka3-morpholinos. (B) RT-PCR analysis of the rps6ka3 MO-injected embryos was conducted to ensure knockdown efficiency. (C) Body curvature was observed in the morphants. (D) Percent distribution of normal embryos vs. embryos with axial deformity, or dead embryos. N?100.

PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Physiol. Biochem.
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