Genomic Feature
it302
- ID
- ZDB-ALT-210609-2
- Name
- it302
- Synonyms
- None
- Affected Genomic Region
- Construct
- None
- Type
- Allele with one delins (1)
- Protocol
- embryos treated with CRISPR
- Lab of Origin
- Kobayashi Lab
- Current Source
- Other Pages
-
Notes
No data available
Variants
- Variant Type
- Delins
- Variant Location
- Chr 2: 41847176 - 41847198 (GRCz11) (1) Details
- Nucleotide change
- AGACCTCGCTCTCGCAGAGCAC/GCAAGGCCTGCTTGTCTACGCAGGCCTTGCAG
- Variant Notes
- None
Effect on DNA/cDNA, transcript, protein (from publications)
- DNA/cDNA Change
- 32 bp inserted / 22 bp deleted in Exon 4 (1)
- Transcript Consequence
- Frameshift (1)
- Protein Consequence
- Polypeptide Truncation (1)
- Flanking Sequence
-
CTCGGTTTCTGGGTGTGTTGAGCGGAGCCAGCTGGGTCCACTGGTTAGTCATGGGGTTGTAGCAGGACAAAGATCCAGACTCTGTGTTGTTCTGCAGGGAGAGATTGCGTCCGCCGACTGTATAAAGAAGCCCGAACAAAACACACGCCCCAAGCCCGCTACAAGGAGACATCATGCTACCTAGTTTGAGCCAGACGTTCTTGTGCGGGTCGAAGGCCTCCAAGGTGTCCAGAGAGTGTTGCTTGTAACCTCCCGCTATGTAAATGAGCTGCGTCCCACGATGAGGTGTTGGCGGCAGGGGTTTTCGGAGAGCCATTTCATGGAAGATCTTTGATAGGAAGTCTTTGCAGGAGTTGGCCTTGCTGAGGATGGGGCAGGACTGCAGTTGTCTTTTGAGGAAAGTGGGTGGAAGGGCGTAGATGTGGACGGCATTGAGGAGGGCATGGAAGTACTGCGCACGGCTCTCTGCGTCCCAGCGTACCCAGTCTATGCAGGCCTTG
AGACCTCGCTCTCGCAGAGCAC/GCAAGGCCTGCTTGTCTACGCAGGCCTTGCAG CTTCAGACTGTCCTGACTGATCAGCTCAAGCAGCTGGCAATGGGACAAGCTGAAGAACTCTTCTTCTTTGGTTACCTGTGAAACACATACATGGAAGATATTTAGCTAGGACATAGTTAAAAACAATTACATTTTCTTACCATTTATTTTTTTGTATCTGTTAAAAACAAAAGCTGATATTCTAATGAGTATTGAAATCCAGCAGCCACTGCCATCCATATTAGGAATAATATGTACTATTGAGGTCAATGGCTGCTGCTTTCCAACAATTTCAACATGTCTTCCTTTGTGTTCAACAGAAAACTCAAACGGGTTTTGGCAAATGGGCAAGGTGAGTAAATAATGACAGAAATTTCATTTCTATCCCTTTTACATCAACCTGCACGGTTAAGCTCCAACCCTAATCAAACACACTTTCTTATAGAATTCTTGTGATCTTAAAGACACTGATTAGCATGTTCAGGTGTGTTTGACTAGTGTTGGAACAAAACTCTGCAGGG - Additional Sequence
- None
Fish
| Fish | Genomic Feature Zygosity | Parental Zygosity | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|---|---|
| keap1ait302/it302 (AB) | Homozygous | ♀+/- ♂+/- | 5 figures  from 2 publications | 2 figures from 2 publications | |
| keap1ait302/+ (AB) | Heterozygous | ♀+/- ♂+/- | Fig. 1 from Bian et al., 2023 | ||
| keap1ait302/+ (AB) | Heterozygous | Unknown | Fig. 4 from Nguyen et al., 2020 | ||
| keap1bit308/it308; keap1ait302/it302 (AB) | Complex | 3 figures from Bian et al., 2023 | 2 figures from Bian et al., 2023 | ||
| keap1bit308/it308; keap1ait302/+ (AB) | Complex | Fig. 1 from Bian et al., 2023 | |||
| keap1bit308/+; keap1ait302/it302 (AB) | Complex | 3 figures from Bian et al., 2023 | Fig. 3 from Bian et al., 2023 | ||
| keap1bit308/+; keap1ait302/+ (AB) | Complex | Fig. 1 from Bian et al., 2023 | |||
| nfe2l2afh318/fh318; keap1ait302/it302 (AB) | Complex | Fig. 2 from Bian et al., 2023 | Fig. 3 from Bian et al., 2023 | ||
| nfe2l2afh318/fh318; keap1ait302/it302 (AB) | Complex | Fig. 4 from Nguyen et al., 2020 | |||
| nfe2l2afh318/+; keap1ait302/it302 (AB) | Complex | Fig. 2 from Bian et al., 2023 | Fig. 3 from Bian et al., 2023 |
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Supplemental Information
- Genotyping protocol
- None
- Bian, L., Nguyen, V.T., Tamaoki, J., Endo, Y., Dong, G., Sato, A., Kobayashi, M. (2023) Genetic hyperactivation of Nrf2 causes larval lethality in Keap1a and Keap1b-double-knockout zebrafish. Redox Biology. 62:102673102673
- Nguyen, V.T., Bian, L., Tamaoki, J., Otsubo, S., Muratani, M., Kawahara, A., Kobayashi, M. (2020) Generation and characterization of keap1a- and keap1b-knockout zebrafish. Redox Biology. 36:101667
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