Tg(ROX-LOXP-LOX2272:2A-mRFP,Xla.Cryg:BFP-LOX2272-LOXP-ROX)

ID

ZDB-TGCONSTRCT-231102-5

Name

Tg(ROX-LOXP-LOX2272:2A-mRFP,Xla.Cryg:BFP-LOX2272-LOXP-ROX)

Previous Names

• UFlip (1)

Type

engineered_plasmid

Regulatory Regions

• LOX2272 (1)
• LOXP (1)
• ROX (1)

Coding Sequences

• 2A (1)
• BFP (1)
• LOX2272 (1)
• LOXP (1)
• mRFP (1)
• ROX (1)

Contains Sequences

None

Note

This construct is flanked by cloning sites for homology arms (HA) complementary to a genomic CRISPR target site, and universal gRNA sites (UgRNA) for in vivo liberation of the targeting cassette. Gene ?off? alleles are generated by integration of the UFlip cassette into an intron in the active orientation, leading to transcription termination and splicing of the primary transcript in the mRFP gene trap. Gene ?on? alleles are generated by integration of the UFlip cassette into an intron in the passive orientation.