Transgenic Construct
Tg(hsp70l:LOXP-STOP-LOXP-pdha1a-2A-mCherry,cryaa:Cerulean)
- ID
- ZDB-TGCONSTRCT-210824-3
- Name
- Tg(hsp70l:LOXP-STOP-LOXP-pdha1a-2A-mCherry,cryaa:Cerulean)
- Previous Names
-
- Tg(cryaa:Cerulean,hsp70l:LOXPSTOP- LOXP-pdha1aSTA-T2A-mCherry) (1)
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- [Em λ][Ex λ]
- Fpbase:mCherry Fpbase:Cerulean
- Contains Sequences
- None
- Note
-
To impair glycolysis specifically in CMs, we used a form of Pdha1a (Pdha1aSTA), which cannot be phosphorylated by Pdk, leading to enhanced pyruvate conversion to acetyl-CoA.
Plasmid Map
None
Genomic Features
That Utilize tg(hsp70l:loxp-stop-loxp-pdha1a-2a-mcherry,cryaa:cerulean) Construct
| Genomic Feature | Affected Genomic Regions |
|---|---|
| bns343Tg |
Transgenics
That Utilize tg(hsp70l:loxp-stop-loxp-pdha1a-2a-mcherry,cryaa:cerulean) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| bns343Tg; pd10Tg |
|
Fig. 3 from Fukuda et al., 2020 |
Sequence Information
Other Construct Pages
None
Citations