Transgenic Construct
TgBAC(cyp1a:NLS-EGFP)
- ID
- ZDB-TGCONSTRCT-150928-7
- Name
- TgBAC(cyp1a:NLS-EGFP)
- Previous Names
- None
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- [Em λ][Ex λ]
- Fpbase:EGFP
- Contains Sequences
- None
- Note
-
The transgene was constructed by modifying a cyp1a-containing fosmid clone using Escherichia coli-based homologous ET-recombination. Fosmid clone (zFD385m15; aka CH1073-385m15) contains 8 kb of upstream sequence and 30 kb of downstream sequence of cyp1a. After recombination, the modified BAC clone contained an nuclear enhanced green fluorescent protein (egfp) gene positioned at an endogenous start site.
Plasmid Map
None
Genomic Features
That Utilize TgBAC(cyp1a:NLS-EGFP) Construct
| Genomic Feature | Affected Genomic Regions |
|---|---|
| cms2Tg |
Transgenics
That Utilize TgBAC(cyp1a:NLS-EGFP) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| cms2Tg/+ (AB) |
|
Sequence Information
Other Construct Pages
None
Citations