Transgenic Construct
Tg(UAS:GCaMP1.6)
- ID
- ZDB-TGCONSTRCT-110912-1
- Name
- Tg(UAS:GCaMP1.6)
- Previous Names
-
- Tg(UAS:GCAMP)
- Tg(UAS:GCaMP)
- tg(uas:gcamp1.6)
- Tg(UAS:GCAMP1.6)
- Tg(UAS:Gga.Mylk-cpEGFP-Rno.Calm2)
- Type
- engineered_plasmid
- Regulatory Regions
- None
- Coding Sequences
- [Em λ][Ex λ]
- Fpbase:EGFP
- Contains Sequences
- Note
-
This construct contains the M13 fragment of the chicken Mylk gene (which contains the target sequence for calmodulin binding), fused to the N terminus of a circularly permutated form of EGFP. The C terminus of cpEGFP is fused to the rat calmodulin gene (CaM). When Ca2+ binds to CaM, the interaction between Ca2+CaMM13 induces a conformational change in cpEGFP which causes a subsequent change in fluorescence intensity.
Plasmid Map
None
Genomic Features
That Utilize Tg(UAS:GCaMP1.6) Construct
| Genomic Feature | Affected Genomic Regions |
|---|---|
| s1993tTg |
Transgenics
That Utilize Tg(UAS:GCaMP1.6) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| bk201Tg; s1993tTg |
|
Fig. 3 from Demaria et al., 2013 | |
| mde4Tg; s1993tTg |
|
2 figures from Demaria et al., 2013 | |
| mde4Tg; s1993tTg + MO1-vmn2r1 |
|
Fig. 7 from Demaria et al., 2013 | Fig. 7 from Demaria et al., 2013 |
| p209Tg; s1993tTg |
|
3 figures from Demaria et al., 2013 | |
| p209Tg; s1993tTg + MO1-vmn2r1 |
|
Fig. 6 from Demaria et al., 2013 | Fig. 6 from Demaria et al., 2013 |
| s1992tTg; s1993tTg |
|
Fig. 5 from Wang et al., 2016 | |
| s1992tTg; s1993tTg + MO1-vit |
|
Fig. 5 from Wang et al., 2016 | |
| s1993tTg |
|
||
| s1993tTg (TLN) |
|
||
| s1993tTg; ub43Tg |
|
Sequence Information
Other Construct Pages
None
Citations