Transgenic Construct
Tg(Xla.Ef1a:GAL4-VP16,hsp70l:Cre,UAS:NLS-RFP)
- ID
- ZDB-TGCONSTRCT-101026-1
- Name
- Tg(Xla.Ef1a:GAL4-VP16,hsp70l:Cre,UAS:NLS-RFP)
- Previous Names
-
- Tg(MAZe) (1)
- Tg(Xla.Ef1a:GAL4-VP16, hsp70l:Cre, UAS:NLS-RFP)
- Tg(Xla.Ef1a:Gal4-VP16, hsp70l:Cre, UAS:nlsRFP)
- Tg(Xla.Ef1a:GAL4-VP16, hsp70l:Cre, UAS:nlsRFP)
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- Contains Sequences
- Note
-
The MAZe transgene was constructed around a self-excising Cre cassette in which expression of Cre recombinase is under the control of the hsp70l promoter. In this system, heat shock activates expression of Cre protein, which induces recombination between flanking loxP sites. Excision of the Cre cassette permits the Xenopus laevis Ef1a promoter to drive expression of Gal4-VP16. Gal4-VP16 activates expression of UAS:nlsRFP.
Plasmid Map
None
Genomic Features
That Utilize Tg(Xla.Ef1a:GAL4-VP16,hsp70l:Cre,UAS:NLS-RFP) Construct
| Genomic Feature | Affected Genomic Regions |
|---|---|
| cj3Tg |
Transgenics
That Utilize Tg(Xla.Ef1a:GAL4-VP16,hsp70l:Cre,UAS:NLS-RFP) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| cj3Tg |
|
2 figures from Collins et al., 2010 | |
| cj3Tg/+ (AB) |
|
||
| cj3Tg; kca3Tg |
|
2 figures from Collins et al., 2010 | |
| cj3Tg; u300Tg |
|
Fig. 4 from Collins et al., 2010 | |
| mlphaj120/+; cj3Tg |
Sequence Information
Other Construct Pages
None
Citations