ZFIN ID: ZDB-PUB-990315-8
Characterisation of a second patched gene in the zebrafish Danio rerio and the differential response of patched genes to hedgehog signalling
Lewis, K.E., Concordet, J.P., and Ingham, P.W.
Date: 1999
Source: Developmental Biology   208: 14-29 (Journal)
Registered Authors: Ingham, Philip, Lewis, Katharine E.
Keywords: hedgehog; signalling; patched2; receptor; zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Embryonic Development
  • Gene Expression Regulation, Developmental/genetics
  • Hedgehog Proteins
  • Membrane Proteins/chemistry
  • Membrane Proteins/genetics*
  • Microinjections
  • Molecular Sequence Data
  • Mutation
  • Phylogeny
  • Proteins/genetics*
  • RNA, Antisense/genetics
  • RNA, Messenger/metabolism
  • Receptors, Cell Surface
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Signal Transduction/genetics
  • Trans-Activators*
  • Transcription, Genetic/genetics
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed: 10075838 Full text @ Dev. Biol.
Hedgehog signalling has been implicated in a variety of processes in vertebrate development, and in each case, the activity of Hh proteins is thought to be mediated by their interaction with a large multipass transmembrane protein encoded by the patched (ptc) gene. In this study we present the full-length coding sequence and describe the wild-type expression pattern of a second ptc gene in zebrafish, Zf-ptc2. We find that at the sequence level Zf-ptc2 is more closely related than Zf-ptc1 to the ptc genes initially characterised in other vertebrate species. We also show that transcription of Zf-ptc2, like Zf-ptc1, is dependent upon Hh signalling and present evidence that it is activated in response to lower levels of Hh activity than is Zf-ptc1. In addition we find no evidence for any specificity in the regulatory interactions between the various Hh proteins and the two ptc genes in the zebrafish.