ZFIN ID: ZDB-PUB-990107-8
Function of zebrafish ß-catenin and TCF-3 in dorsoventral patterning
Pelegri, F. and Maischein, H.M.
Date: 1998
Source: Mechanisms of Development   77: 63-74 (Journal)
Registered Authors: Maischein, Hans-Martin, Pelegri, Francisco
Keywords: Amino Acid Sequence; Animal; Binding Sites; Body Patterning; Cloning, Molecular; Cytoskeletal Proteins; Embryo, Nonmammalian; Gastrula; Gene Expression Regulation, Developmental; Genes, Structural; Homeodomain Proteins; Molecular Sequence Data; Mutation; Sequence Homology, Amino Acid; Support, Non-U.S. Gov't; Transcription Factors; Zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Bacterial Proteins*
  • Binding Sites/genetics
  • Body Patterning/genetics
  • Body Patterning/physiology*
  • Cloning, Molecular
  • Cytoskeletal Proteins/physiology*
  • Drosophila Proteins*
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development
  • Gastrula/metabolism
  • Gene Expression Regulation, Developmental
  • Genes/genetics
  • HMGB Proteins*
  • Homeodomain Proteins/genetics
  • Molecular Sequence Data
  • Mutation
  • Sequence Homology, Amino Acid
  • TCF Transcription Factors
  • Trans-Activators*
  • Transcription Factor 7-Like 1 Protein
  • Transcription Factors/genetics
  • Transcription Factors/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins
  • beta Catenin
PubMed: 9784608 Full text @ Mech. Dev.
We report the molecular cloning and expression of the zebrafish tcf-3 homologue and study its function and that of zebrafish betacat in dorsoventral patterning. Overexpression of mutant zTcf-3 products and Cadherin leads to a reduction in the expression of the dorsal-specific genes goosecoid and chording at the blastula stages, indicating a conserved role for betacat and tcf-3 in zebrafish dorsal axis induction. Later during gastrulation, overexpression of these same products leads to the ectopic expression of dorsal-specific genes in the marginal zone and the induction of ectopic axes, suggesting an additional role for betacat and Tcf-3 at these later stages in the repression of dorsal fates.