PUBLICATION

Overexpression of the forebrain-specific homeobox gene six3 induces rostral forebrain enlargement in zebrafish

Authors

Kobayashi, M., Toyama, R., Takeda, H., Dawid, I.B., and Kawakami, K.

ID

ZDB-PUB-980902-1

Date

1998

Source

Development (Cambridge, England) 125: 2973-2982 (Journal)

Registered Authors

Dawid, Igor B., Kobayashi, Makoto, Takeda, Hiroyuki, Toyama, Reiko

Keywords

sine oculis; six3; homeobox; rostral forebrain; zebrafish; optic stalk

MeSH Terms

Nerve Tissue Proteins/biosynthesis*
Nerve Tissue Proteins/genetics
Drosophila Proteins*
Molecular Sequence Data
Sequence Homology, Amino Acid
Eye Proteins/genetics
Genes, Homeobox*
Prosencephalon/abnormalities*
Prosencephalon/embryology*
Head/abnormalities
Gastrula
Animals
Zebrafish/embryology
Genetic Markers
Amino Acid Sequence
Gene Expression
Homeodomain Proteins/biosynthesis*
Homeodomain Proteins/genetics

(all 18)

PubMed

9655819 Full text @ Development

Abstract

The Drosophila homeobox gene sine oculis is expressed in the rostral region of the embryo in early development and is essential for eye and brain formation. Its murine homolog, Six3, is expressed in the anterior neural plate and eye anlage, and may have crucial functions in eye and brain development. In this study, we describe the cloning and expression of zebrafish six3, the apparent ortholog of the mouse Six3 gene. Zebrafish six3 transcripts are first seen in hypoblast cells in early gastrula embryos and are found in the anterior axial mesendoderm through gastrulation. six3 expression in the head ectoderm begins at late gastrula. Throughout the segmentation period, six3 is expressed in the rostral region of the prospective forebrain. Overexpression of six3 in zebrafish embryos induced enlargement of the rostral forebrain, enhanced expression of pax2 in the optic stalk and led to a general disorganization of the brain. Disruption of either the Six domain or the homeodomain abolish these effects, implying that these domains are essential for six3 gene function. Our results suggest that the vertebrate Six3 genes are involved in the formation of the rostral forebrain.

Genes / Markers

Figures

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Expression

Fish	Conditions	Stage	Anatomy	Assay	Figure
WT	standard conditions	Long-pec	adenohypophysis	ISH	Fig. 4
WT	standard conditions	Long-pec	adenohypophysis	ISH	Fig. 4
WT	standard conditions	Bud	axial hypoblast anterior region	ISH	Fig. 2
WT	standard conditions	75%-epiboly	axial hypoblast anterior region	ISH	Fig. 2
WT	standard conditions	1-4 somites	axial mesoderm	ISH	Fig. 3
WT	standard conditions	Bud	ectoderm anterior region	ISH	Fig. 2
WT	standard conditions	5-9 somites	ectodermal cell	ISH	Fig. 3
WT	standard conditions	1-4 somites	ectodermal cell	ISH	Fig. 3
WT	standard conditions	Prim-5	forebrain anterior surface	ISH	Fig. 4
WT	standard conditions	1-4 somites	forebrain neural keel	ISH	Fig. 2

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Phenotype

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Kobayashi et al., 1998 ZDB-PUB-980902-1 PMID:9655819

Overexpression of the forebrain-specific homeobox gene six3 induces rostral forebrain enlargement in zebrafish

Kobayashi et al., 1998

ZDB-PUB-980902-1
PMID:9655819