PUBLICATION

The molecular nature of zebrafish swirl: BMP2 function is essential during early dorsoventral patterning

Authors
Kishimoto, Y., Lee, K.H., Zon, L., Hammerschmidt, M., and Schulte-Merker, S.
ID
ZDB-PUB-980105-2
Date
1997
Source
Development (Cambridge, England)   124: 4457-4466 (Journal)
Registered Authors
Hammerschmidt, Matthias, Kishimoto, Yasuyuki, Lee, Kyu-Ho, Schulte-Merker, Stefan, Zon, Leonard I.
Keywords
zebrafish; mutation; swirl; BMP2; dorsoventral patterning
MeSH Terms
  • Alleles
  • Animals
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins/genetics*
  • Bone Morphogenetic Proteins/metabolism
  • DNA Primers/genetics
  • Female
  • Genetic Linkage
  • Glycoproteins/genetics
  • Heart/embryology
  • Hematopoiesis/genetics
  • Intercellular Signaling Peptides and Proteins*
  • Male
  • Mesoderm/cytology
  • Mice
  • Mice, Knockout
  • Mutation*
  • Phenotype
  • RNA, Messenger/genetics
  • Transforming Growth Factor beta*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins
PubMed
9409664
Abstract
Early dorsoventral pattern formation in vertebrate embryos is regulated by opposing activities of ventralizing bone morphogenetic proteins (BMPs) and dorsal-specific BMP antagonists such as Chordin, Noggin and Follistatin. Specific defects in early dorsoventral patterning have been recently found in a number of zebrafish mutants, which exhibit either a ventralized or dorsalized phenotype. One of these, the ventralized mutant chordino (originally called dino) is caused by a mutation in the zebrafish chordin homologue and interacts genetically with the dorsalized mutant swirl. In swirl mutant embryos, dorsal structures such as notochord and somites are expanded while ventral structures such as blood and nephros are missing. Here we demonstrate that the swirl phenotype is caused by mutations in the zebrafish bmp2 gene (zbmp2). While injection of mRNAs encoded by the mutant alleles has no ventralizing effect, injection of wild-type zbmp2 mRNA leads to a complete rescue of the swirl mutant phenotype. Fertile adult mutant fish were obtained, showing that development after gastrulation is not dependent on zbmp2 function. In addition zBMP2 has no maternal role in mesoderm induction. Our analysis shows that swirl/BMP2, unlike mouse BMP2 but like mouse BMP4, is required for early dorsoventral patterning of the zebrafish embryo.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes