PUBLICATION
Cleavage of the BMP-4 antagonist chordin by zebrafish tolloid
- Authors
- Blader, P., Rastegar, S., Fischer, N., and Strähle, U.
- ID
- ZDB-PUB-980105-1
- Date
- 1997
- Source
- Science (New York, N.Y.) 278(5345): 1937-1940 (Journal)
- Registered Authors
- Blader, Patrick, Fischer, Nadine, Rastegar, Sepand, Strähle, Uwe
- Keywords
- none
- MeSH Terms
-
- Tolloid-Like Metalloproteinases
- Xenopus Proteins
- Zebrafish Proteins
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- COS Cells
- Drosophila Proteins*
- Glycoproteins/metabolism*
- Transfection
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Gene Expression Regulation, Developmental
- Signal Transduction
- Embryo, Nonmammalian/metabolism
- Bone Morphogenetic Protein 4
- Animals
- Bone Morphogenetic Proteins/antagonists & inhibitors
- Bone Morphogenetic Proteins/metabolism*
- Bone Morphogenetic Protein Receptors
- Insect Proteins/genetics
- Insect Proteins/metabolism*
- Receptors, Growth Factor*
- Receptors, Cell Surface/metabolism
- Intercellular Signaling Peptides and Proteins*
- Cell Lineage
- Body Patterning*
- PubMed
- 9395394 Full text @ Science
Citation
Blader, P., Rastegar, S., Fischer, N., and Strähle, U. (1997) Cleavage of the BMP-4 antagonist chordin by zebrafish tolloid. Science (New York, N.Y.). 278(5345):1937-1940.
Abstract
Dorsoventral patterning of vertebrate and Drosophila embryos requires bone morphogenetic proteins (BMPs) and antagonists of BMP activity. The Drosophila gene tolloid encodes a metalloprotease similar to BMP-1 that interacts genetically with decapentaplegic, the Drosophila homolog of vertebrate BMP-2/4. Zebrafish embryos overexpressing a zebrafish homolog of tolloid were shown to resemble loss-of-function mutations in chordino, the zebrafish homolog of the Xenopus BMP-4 antagonist Chordin. Furthermore, Chordin was degraded by COS cells expressing Tolloid. These data suggest that Tolloid antagonizes Chordin activity by proteolytically cleaving Chordin. A conserved function for zebrafish and Drosophila Tolloid during embryogenesis is proposed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping