|ZFIN ID: ZDB-PUB-980105-1|
Cleavage of the BMP-4 antagonist chordin by zebrafish tolloid
Blader, P., Rastegar, S., Fischer, N., and Strähle, U.
|Source:||Science (New York, N.Y.) 278(5345): 1937-1940 (Journal)|
|Registered Authors:||Blader, Patrick, Fischer, Nadine, Rastegar, Sepand, Strähle, Uwe|
|PubMed:||9395394 Full text @ Science|
Blader, P., Rastegar, S., Fischer, N., and Strähle, U. (1997) Cleavage of the BMP-4 antagonist chordin by zebrafish tolloid. Science (New York, N.Y.). 278(5345):1937-1940.
ABSTRACTDorsoventral patterning of vertebrate and Drosophila embryos requires bone morphogenetic proteins (BMPs) and antagonists of BMP activity. The Drosophila gene tolloid encodes a metalloprotease similar to BMP-1 that interacts genetically with decapentaplegic, the Drosophila homolog of vertebrate BMP-2/4. Zebrafish embryos overexpressing a zebrafish homolog of tolloid were shown to resemble loss-of-function mutations in chordino, the zebrafish homolog of the Xenopus BMP-4 antagonist Chordin. Furthermore, Chordin was degraded by COS cells expressing Tolloid. These data suggest that Tolloid antagonizes Chordin activity by proteolytically cleaving Chordin. A conserved function for zebrafish and Drosophila Tolloid during embryogenesis is proposed.