PUBLICATION

Cleavage of the BMP-4 antagonist chordin by zebrafish tolloid

Authors
Blader, P., Rastegar, S., Fischer, N., and Strähle, U.
ID
ZDB-PUB-980105-1
Date
1997
Source
Science (New York, N.Y.)   278(5345): 1937-1940 (Journal)
Registered Authors
Blader, Patrick, Fischer, Nadine, Rastegar, Sepand, Strähle, Uwe
Keywords
none
MeSH Terms
  • Animals
  • Body Patterning*
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Protein Receptors
  • Bone Morphogenetic Proteins/antagonists & inhibitors
  • Bone Morphogenetic Proteins/metabolism*
  • COS Cells
  • Cell Lineage
  • Drosophila Proteins*
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental
  • Glycoproteins/metabolism*
  • Insect Proteins/genetics
  • Insect Proteins/metabolism*
  • Intercellular Signaling Peptides and Proteins*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Receptors, Cell Surface/metabolism
  • Receptors, Growth Factor*
  • Signal Transduction
  • Tolloid-Like Metalloproteinases
  • Transfection
  • Xenopus Proteins
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins
PubMed
9395394 Full text @ Science
Abstract
Dorsoventral patterning of vertebrate and Drosophila embryos requires bone morphogenetic proteins (BMPs) and antagonists of BMP activity. The Drosophila gene tolloid encodes a metalloprotease similar to BMP-1 that interacts genetically with decapentaplegic, the Drosophila homolog of vertebrate BMP-2/4. Zebrafish embryos overexpressing a zebrafish homolog of tolloid were shown to resemble loss-of-function mutations in chordino, the zebrafish homolog of the Xenopus BMP-4 antagonist Chordin. Furthermore, Chordin was degraded by COS cells expressing Tolloid. These data suggest that Tolloid antagonizes Chordin activity by proteolytically cleaving Chordin. A conserved function for zebrafish and Drosophila Tolloid during embryogenesis is proposed.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping