PUBLICATION

Notochord induction of zebrafish slow muscle mediated by Sonic hedgehog

Authors
Blagden, C.S., P.D. Currie, P.W. Ingham, and S.M. Hughes
ID
ZDB-PUB-971103-2
Date
1997
Source
Genes & Development   11(17): 2163-2175 (Journal)
Registered Authors
Blagden, Chris, Currie, Peter D., Hughes, Simon M., Ingham, Philip
Keywords
zebrafish; muscle; fiber type; adaxial cells; sonic hedgehog; myoblast
MeSH Terms
  • Animals
  • Biological Evolution
  • Embryonic Induction/genetics
  • Embryonic Induction/physiology*
  • Hedgehog Proteins
  • Immunohistochemistry
  • Mesoderm/cytology
  • Muscle Fibers, Fast-Twitch/cytology
  • Muscle Fibers, Fast-Twitch/metabolism
  • Muscle Fibers, Slow-Twitch/cytology*
  • Muscle Fibers, Slow-Twitch/metabolism
  • Mutation
  • Myosin Heavy Chains/metabolism
  • Notochord/embryology*
  • Proteins/genetics
  • Proteins/physiology*
  • Time Factors
  • Trans-Activators*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed
9303533 Full text @ Genes & Dev.
Abstract
The patterning of vertebrate somitic muscle is regulated by signals from neighboring tissues. We examined the generation of slow and fast muscle in zebrafish embryos and show that Sonic hedgehog (Shh) secreted from the notochord can induce slow muscle from medial cells of the somite. Slow muscle derives from medial adaxial myoblasts that differentiate early, whereas fast muscle arises later from a separate myoblast pool. Mutant fish lacking shh expression fail to form slow muscle but do form fast muscle. Ectopic expression of shh, either in wild-type or mutant embryos, leads to ectopic slow muscle at the expense of fast. We suggest that Shh acts to induce myoblasts committed to slow muscle differentiation from uncommitted presomitic mesoderm.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes