PUBLICATION

A hemophilia model in zebrafish: Analysis of hemostasis

Authors
Jagadeeswaran, P. and Liu, Y.C.
ID
ZDB-PUB-970314-8
Date
1997
Source
Blood cells, molecules & diseases   23(3): 52-57 (Journal)
Registered Authors
Jagadeeswaran, Pudur
Keywords
hemophilia; zebrafish; hemostasis; clotting time; copper; mutagenesis
MeSH Terms
  • Animals
  • Blood Coagulation/drug effects
  • Copper/toxicity
  • Disease Models, Animal*
  • Hemophilia A/chemically induced
  • Hemophilia A/physiopathology*
  • Hemostasis*
  • Zebrafish*
PubMed
9215750 Full text @ Blood Cells Mol. Dis.
Abstract
We have developed artificial hemophilia in zebrafish by treating them with copper and measured their clotting function by a newly developed sensitive clotting time assay. The clotting function can be detected rapidly and reliably in 30 hr larvae and in adult fish by measuring the blood clotting time. We have used this assay to screen wild type zebrafish and identified fish with prolonged clotting time. This verifies the usefulness of this assay in future screening for recessive hemostasis defects generated by chemical and radiation mutagenesis methods.
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Mutations / Transgenics
Human Disease / Model
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Fish
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Mapping