PUBLICATION
A hemophilia model in zebrafish: Analysis of hemostasis
- Authors
- Jagadeeswaran, P. and Liu, Y.C.
- ID
- ZDB-PUB-970314-8
- Date
- 1997
- Source
- Blood cells, molecules & diseases 23(3): 52-57 (Journal)
- Registered Authors
- Jagadeeswaran, Pudur
- Keywords
- hemophilia; zebrafish; hemostasis; clotting time; copper; mutagenesis
- MeSH Terms
-
- Animals
- Blood Coagulation/drug effects
- Copper/toxicity
- Disease Models, Animal*
- Hemophilia A/chemically induced
- Hemophilia A/physiopathology*
- Hemostasis*
- Zebrafish*
- PubMed
- 9215750 Full text @ Blood Cells Mol. Dis.
Citation
Jagadeeswaran, P. and Liu, Y.C. (1997) A hemophilia model in zebrafish: Analysis of hemostasis. Blood cells, molecules & diseases. 23(3):52-57.
Abstract
We have developed artificial hemophilia in zebrafish by treating them with copper and measured their clotting function by a newly developed sensitive clotting time assay. The clotting function can be detected rapidly and reliably in 30 hr larvae and in adult fish by measuring the blood clotting time. We have used this assay to screen wild type zebrafish and identified fish with prolonged clotting time. This verifies the usefulness of this assay in future screening for recessive hemostasis defects generated by chemical and radiation mutagenesis methods.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping