PUBLICATION
Spatial patterns of olfactory neurons expressing specific odor receptor genes in 48-hour-old embryos of zebrafish, Danio rerio
- Authors
- Vogt, R.G., Lindsay, S.M., Byrd, C.A., and Sun, M.
- ID
- ZDB-PUB-970129-5
- Date
- 1997
- Source
- The Journal of experimental biology 200: 433-443 (Journal)
- Registered Authors
- Byrd-Jacobs, Christine, Sun, Ming, Vogt, Richard
- Keywords
- odor receptor; pattern formation; zebrafish; embryogenesis; gene expression; Danio rerio
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental*
- Olfactory Receptor Neurons/metabolism*
- Receptors, Odorant/genetics*
- Receptors, Odorant/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 9057306 Full text @ J. Exp. Biol.
Citation
Vogt, R.G., Lindsay, S.M., Byrd, C.A., and Sun, M. (1997) Spatial patterns of olfactory neurons expressing specific odor receptor genes in 48-hour-old embryos of zebrafish, Danio rerio. The Journal of experimental biology. 200:433-443.
Abstract
Olfactory neurons have a complex phenotype characterized by their expression of a specific odor receptor (OR) gene and their targeting of an equally specific locus in the olfactory bulb. In the adult fish, olfactory neurons expressing specific ORs are broadly distributed in the epithelium, intermingling with neurons expressing other OR phenotypes. This distributed adult pattern has led to the suggestion that olfactory neuron phenotype is determined by a stochastic process, independent of external positional cues. However, when the fish olfactory system is established during embryogenesis it is simple in its organization, with few olfactory neurons and an olfactory epithelium that has not yet folded into the adult morphology. It is possible that positional cues might act in the embryo to establish an initial population and pattern of olfactory neuron phenotypes and that subsequent morphogenesis and neuronal addition lead to the randomized distribution of neurons. To test this possibility, we examined the spatial patterns of olfactory neurons expressing specific OR genes in 48 h embryos, a time of relative simplicity in the developing olfactory epithelium. Three-dimensional plots of neuron distributions were made, and comparison of OR expression patterns were made between right and left epithelia, between individual animals and between different OR genes. The patterns of OR gene expression were not conserved in these comparison. Mathematical analysis of 21 epithelia for the degree of order in the distribution of olfactory neurons argued strongly that the neurons expressing given ORs are randomly distributed in the 48 h embryos. These results are consistent with those observed from adult tissue and support models suggesting that extrinsic positional cues do not have a major role in specifying olfactory neuron phenotypes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping